Antifoam SE-15 - aqueous emulsion for bacterial and mammalian systems

Cat. No.: A489557

GRADE & PURITY aqueous emulsion for bacterial and mammalian systems

Storage

Room temperature

Shipped In

Normal

Descripción general

Antifoam SE-15 is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.

Antifoams are supplied as two basic types of composition or mixtures of the two. Organic antifoams are of synthetic origin. Silicone-based antifoams are generally considered to be siloxane polymers and are also synthetic. Our suppliers generally consider the exact composition to be proprietary.Products  are listed as Molecular Biology Grade and have been tested for use in bacterial fermentation. No significant inhibition is found when E. coli or B. subtilis is grown in Terrific Broth supplemented with effective concentrations of antifoams. These products have individual data sheets available upon request.

Components

Antifoam SE-15 is an aqueous emulsion containing 10% active silicon and non-ionic emulsifiers .

Preparation Note

Antifoam SE-15 can be pre-diluted with 3-10 parts of cool water to aid in dispersion. Pre-diluted suspensions should be used immediately.Sterilize by autoclaving. Multiple autoclaving will not significantly affect performance. The flow properties of Antifoam SE-15 allow pumping to a fermenter on an asneeded basis.

Procedure

Antifoams should be added prior to the point where foaming occurs. Alternatively, antifoam can be used as supplied, by wiping on filling nozzles, rims of processing vats, or on a screen suspended above the foaming system. One of the most common applications for antifoams is in microbiological fermentation.Since one cannot predict the effectiveness of an antifoam for a particular application, antifoams should also be tested to ensure adequate defoaming effectiveness under representative culture conditions for each microorganism. The variables of medium composition, temperature, pH, mixing, and aeration anticipated for the final experimental or fermentation conditions should be used. If the antifoam is not effective under these test conditions either a higher amount of antifoam can be added or a different type of antifoam can be tested.

Applications

Antifoam SE-15 has been used in the preparation of ramified rolling circle amplification (RAM) amplification buffer. It has also been used to find its effects on cell viability, virus infectivity and PCR (polymerase chain reaction) performance.

The application of antifoam silicones in the microbiological fermentation technique.Foam formation and the subsequent cell damage/ losses in the foam layer were found to be the major problems affecting cell growth and monoclonal antibody (MAb) production in stirred and sparged bioreactors for both serum-supplemented and serum-free media. Surfactants in the culture media had a profound effect on cell growth by changing both the properties of bubbles and the qualities of foam formed.

Addition of high concentrations of silicone antifoam to a suspension of hybridoma cells in a bubble column reduces the death rate when using medium without a protective component.The mechanism of antifoaming in a nonionic Tritonä X-100 surfactant solution with silicone polyethers, the so-called "cloud point antifoams," was investigated. The cloud point (CP) studies showed that the CP of a Triton X-100/silicone polyether mixed system is between the CP of the foaming and the antifoam surfactants, due to mixed micelle formation.Supercritical carbon dioxide was used for the direct extraction of drugs from plasma prior to analysis. The supercritical fluid was directly passed through plasma samples spiked with either a neutral (flavone) or an acidic (ketorolac) drug. The addition of an antifoam agent to the plasma prior to extraction was required to avoid restrictor plugging caused by denaturation of theplasma proteins by the supercritical fluid.

Specifications

Especificaciones y pureza

aqueous emulsion for bacterial and mammalian systems

Condiciones de almacenamiento de almacenamiento

Room temperature

Enviado en

Normal

Propiedades químicas y físicas

• Solubilidad: H2O: soluble (effective hot or cold.)