ATP Citrate Lyase (ACL) Activity Assay Kit (UV Micro Method)

Cat. No.: A1515845

GRADE & PURITY BioReagent

Descripción general

ATP Citrate Lyase (ACL) is a key cytoplasmic enzyme that catalyzes the formation of acetyl-CoA from citrate. The acetyl-CoA produced serves as the primary precursor for the synthesis of fatty acids, cholesterol, and other lipids, and it is also involved in the modification of important proteins. As such, acetyl-CoA is a central hub in energy metabolism. This assay kit is suitable for detecting ACL activity in samples such as animal and plant tissues, bacteria, cells, and serum (plasma). In this kit, in the presence of ATP and coenzyme A, ACL catalyzes the cleavage of citrate into acetyl-CoA, oxaloacetate, ADP, and inorganic phosphate. Malate dehydrogenase then further catalyzes the reaction of oxaloacetate with NADH to produce malate and NAD⁺, resulting in a decrease in absorbance at 340 nm.

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Protected from light,Store at -20°C

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20℃ for 6 months. Store in the dark.