Atto 425-NHS ester - ≥90% , CAS No.892156-28-4

CAS: 892156-28-4 Cat. No.: A334800 Peso molecular: 498.52

GRADE & PURITY ≥90%

Synonyms

Atto 425-NHS ester | Atto 425-N-hydroxysuccinimide ester | ATTO 425-3 | ATTO 425 NHS ester ;Atto 425-NHS ester | ethyl 9-{4-[(2,5-dioxopyrrolidin-1-yl)oxy]-4-oxobutyl}-6,8,8-trimethyl-2-oxo-6,7,8,9-tetrahydro-2H-pyrano[3,2-g]quinoline-3-carboxylate

Storage

Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

Descripción general

ATTO 425 NHS ester is a new fluorescent marker based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 425 NHS ester is an NHS ester derivative of ATTO 425 that can be used to label proteins or antibodies.

Application：

Atto 425 NHS ester is used in the development of bifunctional fluorescent, inorganic micro-partices (Zeolite L crystals) designed for targeting or imaging mutliple components . Reacts with amino groups or amine modified oliognucleotides to form a stable amine bond. Utilized as an essential component of molecular beacons, which were designed for use in real-time PCR assays to allow for rapid and accurate identification of gram-negative bacteria . When conjugated with benzylguanine, the fluorescence of Atto 425-NHS is quenched but becomes fluorescent upon labeling of the SNAP-tag fusion protein, making this conjugate an ideal candidate for experiments in living cells . Used as one of the donor probes in three-color FRET experiments designed to investigate the kinetics of RNA-protein complex formation .
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Specifications

Sinónimos

Atto 425-NHS ester | Atto 425-N-hydroxysuccinimide ester | ATTO 425-3 | ATTO 425 NHS ester ;Atto 425-NHS ester | ethyl 9-{4-[(2, 5-dioxopyrrolidin-1-yl)oxy]-4-oxobutyl}-6, 8, 8-trimethyl-2-oxo-6, 7, 8, 9-tetrahydro-2H-pyrano[3, 2-g]quinoline-3-carboxylate

Especificaciones y pureza

≥90%

Condiciones de almacenamiento de almacenamiento

Store at -20°C, Argon charged

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Pureza

≥90%

Propiedades del producto

• Rangos de excitación y emisión: λex 437 nm,λem 483 nm in 0.1 M phosphate pH 7.0

Nombres e identificadores

• Pubchem Sid: 488199097
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/488199097
• Sonrisas canónicas: CCOC(=O)C1=CC2=CC3=C(C=C2OC1=O)N(C(CC3C)(C)C)CCCC(=O)ON4C(=O)CCC4=O
• IUPAC Name: ethyl 9-[4-(2,5-dioxopyrrolidin-1-yl)oxy-4-oxobutyl]-6,8,8-trimethyl-2-oxo-6,7-dihydropyrano[3,2-g]quinoline-3-carboxylate
• InChIKey: PUEQEMDTFPYCDY-UHFFFAOYSA-N
• INCHI: 1S/C26H30N2O8/c1-5-34-24(32)18-12-16-11-17-15(2)14-26(3,4)27(19(17)13-20(16)35-25(18)33)10-6-7-23(31)36-28-21(29)8-9-22(28)30/h11-13,15H,5-10,14H2,1-4H3
• Isómeros SMILES: CCOC(=O)C1=CC2=CC3=C(C=C2OC1=O)N(C(CC3C)(C)C)CCCC(=O)ON4C(=O)CCC4=O
• WGK Alemania: 3
• Peso molecular: 498.52
• Reaxy-Rn: 54561732
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=54561732&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Clase Benzopyrans
      • Subclass 1-benzopyrans

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Clase: Benzopyrans
• Subclass: 1-benzopyrans
• Intermediate Tree Nodes: Not available
• Direct Parent: Chromenopyridines
• Alternative Parents: Coumarins and derivatives Hydroquinolines Dialkylarylamines Pyranones and derivatives Aralkylamines Pyrrolidine-2-ones Benzenoids Dicarboxylic acids and derivatives Dicarboximides Heteroaromatic compounds Amino acids and derivatives Carboxylic acid esters Carboxylic acid salts Lactones Lactams Azacyclic compounds Oxacyclic compounds Organic salts Organic oxides Organopnictogen compounds Carbonyl compounds Hydrocarbon derivatives
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Chromenopyridine - Coumarin - Tetrahydroquinoline - Tertiary aliphatic/aromatic amine - Dialkylarylamine - Pyranone - Aralkylamine - Dicarboxylic acid or derivatives - Pyran - Pyrrolidone - 2-pyrrolidone - Benzenoid - Dicarboximide - Pyrrolidine - Heteroaromatic compound - Tertiary amine - Lactone - Lactam - Carboxylic acid ester - Carboxylic acid salt - Amino acid or derivatives - Carboxylic acid derivative - Oxacycle - Azacycle - Hydrocarbon derivative - Organopnictogen compound - Organonitrogen compound - Organic oxygen compound - Organic nitrogen compound - Organooxygen compound - Amine - Organic oxide - Carbonyl group - Organic salt - Aromatic heteropolycyclic compound
• Descripción: This compound belongs to the class of organic compounds known as chromenopyridines. These are aromatic heterocyclic compounds structurally characterized by a pyridine ring fused to a chromene moiety.
• External Descriptors: ethyl ester - pyrrolidinone

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Propiedades químicas y físicas

• Solubilidad: Soluble in DMF, DMSO, and water.
• Sensibilidad: Moisture sensitive.
• Peso molecular: 498.500 g/mol
• XLogP3: 3.100
• Hydrogen Bond Donor Count: 0
• Hydrogen Bond Acceptor Count: 9
• Rotatable Bond Count: 9
• Exact Mass: 498.2 Da
• Monoisotopic Mass: 498.2 Da
• Topological Polar Surface Area: 120.000 Ų
• Heavy Atom Count: 36
• Formal Charge: 0
• Complexity: 957.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 1
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

Referencias

1. Jian Liu, Junbo Li, Yan Huang, Tong Li, Cheng Xu, Zhengyu Tao, Wei Ji, Xin Huang.  (2025)  Liquid-to-gel transitions of phase-separated coacervate microdroplets enabled by endogenous enzymatic catalysis.  JOURNAL OF COLLOID AND INTERFACE SCIENCE,      [PMID:40184654] [10.1016/j.jcis.2025.137486]