Blood-Brain Barrier Permeability Assay Kit

Cat. No.: B1506000

Storage

Protected from light,Room temperature

Shipped In

Normal

Application

Cell Staining

Descripción general

The core of the Blood-Brain Barrier (BBB) Permeability Assay Kit lies in using spectrophotometry to detect the concentration of substances that have penetrated the simulated blood-brain barrier. It features simple operation and low cost, making it suitable for rapid screening. No fluorescent labeling or radioactive probes are required; instead, it relies on the inherent light absorption properties of the substances themselves (or supporting chromogenic reagents). It has a low operational threshold and uses widely available equipment (a standard laboratory spectrophotometer is sufficient).

B1506000	Components	50T	Storage
B1506000A	Standard Substance A	2 mL	RT. Store in the dark.
B1506000B	Standard Substance B	2 mL	RT. Store in the dark.
B1506000C	Standard Substance C	2 mL	RT. Store in the dark.
B1506000D	Standard Substance D	2 mL	RT. Store in the dark.
B1506000E	Standard Substance E	2 mL	RT. Store in the dark.
B1506000F	Staining Solution	10 mL	RT
B1506000G	Isolation Solution	50 mL	RT
B1506000H	PBS	1 L	RT

Self-Prepared Consumables and Reagents:

Syringe

Tissue Homogenizer

Operating Steps (for reference only):

a. Take the treated experimental animals (using mice as an example). Intravenously inject 1–200 μl of staining solution (No. F). Within a few seconds to 1 minute, the eyes and skin of the mice will turn blue. Sacrifice the mice after 0.5–1 hour and collect the target brain tissue.

b. Place the brain tissue in a 1.5 ml centrifuge tube, add 1 ml of PBS, quickly homogenize the brain tissue using a tissue homogenizer, and centrifuge at 1000×g for 10 minutes at 4°C.

c. Take the supernatant, add an equal volume of separation solution (No. G), mix well, and incubate at 4°C for 18–24 hours.

d. Centrifuge at 2000×g for 15 minutes at 4°C.

e. Take an appropriate amount of the above solution and dilute it 10-fold. Take 1 ml of the diluted solution and measure the absorbance (OD value) at 620 nm using a spectrophotometer or microplate reader. Meanwhile, determine the OD values of standards A–E, plot a standard curve, and calculate the content of the staining solution in the test samples based on the standard curve.

Measured Values by the Company's Spectrophotometer:

B1506000	Standard Substance A	Standard Substance B	Standard Substance C	Standard Substance D	Standard Substance E
Concentration	39.062mM	9.766mM	2.441mM	0.610mM	0.152mM
OD Value	1.036	0.631	0.234	0.116	0.087

(Only relative changes in permeability can be observed, absolute quantification is not possible)

Precautions:

1. In the blood-brain barrier permeability experiment, the injection volume of the staining solution should be adjusted according to the type of animal and the animal's weight.

2. A low-temperature refrigerated centrifuge should be used for centrifugation.

3. For your safety and health, please wear a lab coat and disposable gloves during operation.

Specifications

Estabilidad y almacenamiento

Store at RT long term (12 months). Store in the dark.

Condiciones de almacenamiento de almacenamiento

Protected from light, Room temperature

Enviado en

Normal

Propiedades químicas y físicas

• Sensibilidad: Light-sensitive