c-Kit/CD117 Rat mAb (FITC) - Primary antibody, specific to c-Kit/CD117

Cat. No.: Ab209973

GRADE & PURITY Validated, ExactAb™, Ex:498nm, Em:517nm, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

ACK2

Conjugation

FITC

Descripción general

Rat anti Mouse c-Kit/CD117 Antibody, Monoclonal (ACK2), could be used for Flow, IP, in vivo mast cell depletion, in vivo c-Kit+ cell depletion, in vitro c-Kit neutralization and so on.

Specifications

Product Name

c-Kit/CD117 Rat mAb (FITC) - Primary antibody, specific to c-Kit/CD117

Sinónimos

W | Bs | Fdc | Ssm | SCO1 | SCO5 | v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody | FITC anti-mouse CD117 (c-kit) Antibody | CD117 (c-Kit) Monoclonal Antibody (ACK2), FITC | FITC Anti-Mouse CD117/c-Kit (ACK2) | v kit Hardy Zuckerma

Especificaciones y pureza

ExactAb™, Validated, Ex:498nm, Em:517nm, 0.5 mg/mL

Mecanismos bioquímicos y fisiológicos

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration a

Especies hospedadoras

Rat

Especificidad

c-Kit/CD117

Inmunógeno

IL-3-dependent mast cells

Control positivo

Flow: BALB/C mouse bone marrow cells

Conjugación

FITC

Excitación (nm)

Blue Laser(488nm)

Emisión (nm)

517nm

Ex/Em(nm)

Ex:498nm;Em:517nm

Grado

Em:517nm, Ex:498nm, ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein G purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: c-Kit/CD117 Rat mAb (FITC) (Ab209973)

c-Kit/CD117 Rat mAb (FITC) (Ab209973) - Flow Cytometry
Two-color flow cytometric analysis of c-Kit/CD117 expression on BALB/C mouse bone marrow cells. BALB/C mouse bone marrow cells were stained with CD45R Rat mAb (AF647) (Ab191663) and either Rat IgG (FITC) (Ab180311; Left panel) or c-Kit/CD117 Rat mAb (FITC) (Ab209973; Right panel) at 0.2μg/test.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤1.0 μg/test, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume ;

Objetivos asociados (humanos)

KIT Tclin Mast/stem cell growth factor receptor Kit (0 Activities)

Discover Target: KIT

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)