Protein Purity
≥85% by SDS PAGE
Extinction Coeff
A280 nm = 0.994 at 1.0 mg/ml for pure C3
Molecular Weight
184,000 Da (2 chains)
General Description
Mouse C3 is purified from pooled normal mouse serum. C3 is central to the activation of all three pathways of complement activation (Law, S.K.A. and Reid, K.B.M. (1995)). Initiation of each pathway generates proteolytic enzyme complexes (C3 convertases) which are bound to the target surface. These enzymes cleave a peptide bond in C3 releasing the anaphylatoxin C3a and activating C3b. For a brief time (~60 µs) this nascent C3b is capable of reacting with and covalently coupling to hydroxyl groups on the target surface. Carbohydrates are the favored target, but protein hydroxyls and amino groups also react. This process of tagging the target surface with C3b is called opsonization. The reactive site in nascent C3b is a thioester (Tack B.J., et al. (1980); Pangburn M.K. and MüllerEberhard H.J. (1980)) and C3b is linked to the target through a covalent ester bond (an amide bond is formed if C3b is attached to amino groups). Most of the C3 activated during complement activation never attaches to the surface because its thioester reacts with water forming fluid phase C3b which is rapidly inactivated by factors H and I forming iC3b. Surface-bound C3b is necessary in all three pathways for efficient activation of C5 and formation of C5b-9 complexes that lyse the target cell membrane. Surface-bound C3b and its breakdown products iC3b and C3d are recognized by numerous receptors on lymphoid and phagocytic cells which use the C3b ligand to stimulate antigen presentation to cells of the adaptive immune system. The end result is an expansion of target-specific B-cell and T-cell populations.
Physical Characteristics & Structure
The calculated molecular weight of mouse C3 based on its amino acid sequence is 184,180 daltons and is similar to that of human C3 (185,000 daltons).The molecular weight of
mouse C3 as determined by SDS/polyacrylamide gel electrophoresis has been reported by Van den Berg, C.W. et al., (1989) to be 170,000 daltons composed of two disulfide linked chains, alpha chain (107,000 daltons) and beta chain (62,000 daltons). The extinction coefficient of mouse C3 is calculated based on its amino acid sequence using ProtParam and assumes all pairs of Cys residues form cystines (i.e. a pair of cysteine molecules are joined by a disulfide bond). The theoretical pI of mouse C3 is 6.3. The normal concentration of mouse C3 in serum has been reported to be 0.7mg/ml (Van den Berg et al., (1989)).
Function
The biological functions of C3 are described above in the General Description and Physical Characteristics sections.
Genetics
Mouse C3 chromosome location 17. The NCBI Gene ID number for mouse C3 is 12266 and UniProt accession number is P01027.
Precautions/Toxicity/Hazards
This protein is purified from animal plasma/serum and therefore precautions appropriate for handling any animal blood-derived product must be used.
References
Law, S.K.A. and Reid, K.B.M. (1995) Complement 2nd Edition (ISBN 0199633568) Oxford University Press, Oxford.Tack BF, Harrison RA, Janatova J, Thomas ML, Prahl JW. (1980) Evidence for presence of an internal thiolester bond in third component of human complement. Proc Natl Acad Sci U S A. 77:5764-8.
Pangburn M.K. and Müller-Eberhard H.J. (1980) Relation of putative thioester bond in C3 to activation of the alternative pathway and the binding of C3b to biological targets of complement. J Exp Med. 152:1102-14.
Van den Berg, C.W., Van Dijk , H. and Capel, P.J.A. (1989). Rapid isolation and characterization of native mouse complement components C3 and C5. J. Immunological Methods. 122:73-78.
