Determine the necessary mass, volume, or concentration for preparing a solution.
BioReagent, suitable for electrophoresis, for PAGE, for western blot, 5× BioReagent,for PAGE,for Western blot,Suitable for electrophoresis para flujos de trabajo cromatográficos y analíticos sensibles que requieren una interferencia mínima de la línea base.
Store at -20°C Envío Ice chest + Ice pads. Consulte el COA específico del lote para conocer las especificaciones exactas.
SDS, COA, ficha técnica y hoja de especificaciones disponibles para descargar. COA específico del lote accesible mediante búsqueda por número de lote.
Citado en 0 publicaciones revisadas por pares sobre cromatografía, síntesis orgánica y reacciones de acoplamiento cruzado.
This product is designed for protein sample preparation in SDS-PAGE electrophoresis. Its novel formulation ensures a neutral pH after boiling, preventing protein degradation. It features fast thawing from –20°C and eliminates unpleasant odors by replacing traditional reducing agents (e.g., β-mercaptoethanol or DTT) with an advanced odorless alternative.
Key Features
1.Prevents Degradation: Neutral pH after boiling avoids protein degradation, ideal for phosphorylated proteins.
2.Rapid Settlement: Samples sink quickly to the bottom of wells without floating.
3.Fast Thawing: Rapid melting from –20°C without viscosity.
4.Odorless: Contains a novel reducing agent—no β-mercaptoethanol or DTT, no unpleasant smell.
Protocol
1.Add 1 volume of loading buffer to 4 volumes of protein sample (1:4 ratio) and mix thoroughly.
2.Heat the mixture in a boiling water bath for 5–10 minutes.
3.Centrifuge at high speed for 5 minutes. Use the supernatant for electrophoresis.
3.1Note: If no insoluble debris is observed after adding loading buffer, brief centrifugation is sufficient to collect the mixture.
3.2If condensation forms on the tube lid after heating, gently flick the tube to mix condensation with the sample before centrifugation to ensure uniform loading concentration.
Precautions
1.Gels loaded with this buffer may exhibit yellowing or streaks in silver staining due to the reducing agent.
2.Not suitable for non-denaturing gel electrophoresis as it contains denaturing agents.
3.Wear a lab coat and disposable gloves for safety.
4.For research use only.
Documento completo sobre peligros, manipulación, almacenamiento y cumplimiento normativo.
<<<<<<< HEAD Descargar SDS → ======= Descargar SDS → >>>>>>> release248-p3Datos de calidad específicos del lote. Introduzca su número de lote para obtener el COA exacto.
Buscar COA →Resumen de referencia rápida de las especificaciones y aplicaciones del producto.
Ver ficha técnica →Atributos de calidad completos y criterios de aceptación para esta calidad.
Ver hoja de especificaciones →Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Fecha | Articulo |
|---|---|---|---|
| Certificate of Analysis | Jul 12, 2026 | S750308 |
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Ver la guía de calidades BioReagent → Ver la guía de calidades Suitable for electrophoresis → Ver la guía de calidades for PAGE → Ver la guía de calidades for Western blot →