CD19 Rat mAb(Biotin) - Primary antibody, specific to CD19, Rat IgG2a

Cat. No.: Ab185392

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

1D3

Conjugation

Biotin

Descripción general

Flow: ≤0.3 μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Specifications

Product Name

CD19 Rat mAb(Biotin) - Primary antibody, specific to CD19, Rat IgG2a

Sinónimos

Biotin Anti-Mouse CD19 Antibody[1D3] | Biotin Anti-CD19 antibody [1D3] | Biotin Rat Anti-Mouse CD19 | Biotin anti-mouse CD19 Antibody | T-cell surface antigen Leu-12 antibody | MGC12802 antibody | MGC109570 antibody | Leu12 antibody | Leu-12 antibody | Le

Especificaciones y pureza

ExactAb™, Validated, 0.5 mg/mL

Especies hospedadoras

Rat

Especificidad

CD19

Inmunógeno

Transfected k562 cells expressing the extracellular domain of murine CD19.

Control positivo

Flow: Mouse splenocytes.

Conjugación

Biotin

Grado

ExactAb™, Validated

Isotipo

Rat IgG2a

Tipo de cadena ligera

kappa

Método de purificación

Protein G purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: CD19 Rat mAb (Biotin) (Ab185392)

CD19 Rat mAb (Biotin) (Ab185392) - Flow Cytometry
Flow cytometry analysis of CD19 expression by mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD19 Rat mAb (Biotin) (Ab185392) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).

Flow Cytometry: CD19 Rat mAb (Biotin) (Ab185392)

CD19 Rat mAb (Biotin) (Ab185392) - Flow Cytometry
Flow cytometry analysis of CD19 expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD19 Rat mAb (Biotin) (Ab185392) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Objetivos asociados (humanos)

CD19 Tclin B-lymphocyte antigen CD19 (0 Activities)

Discover Target: CD19

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción