CD44 Rat mAb - Primary antibody, specific to CD44, Rat IgG2a

Cat. No.: Ab094866

GRADE & PURITY Carrier Free, Validated, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay, IF/ICC, WB

Host species

Rat

Reactive species

Human

Isotype

Rat IgG2a

Clone Number

Hermes-1

Conjugation

Unconjugated

Descripción general

Rat anti Human CD44 Antibody, Monoclonal (Hermes-1), could be used for Flow, in vivo CD44 blockade in xenografts, in vitro CD44 blockade, WB, ICC, IF and so on.
Application:
Flow: Use at an assay dependent concentration.
in vivo CD44 blockade in xenografts: Use at an assay dependent concentration. 
in vitro CD44 blockade: Use at an assay dependent concentration. 
WB: Use at an assay dependent concentration. 
ICC/IF: Use at an assay dependent concentration. 
Protein Function
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.

Specifications

Product Name

CD44 Rat mAb - Primary antibody, specific to CD44, Rat IgG2a

Sinónimos

CDW44 | H-CAM | ECM-III | HUTCH-1 | Hermes-1 | CD44 antigen | CD44 molecule (Indian blood group) | CD44 | CD44R | CDw44 | cell surface glycoprotein CD44 | chondroitin sulfate proteoglycan 8 | CSPG8 | ECMR-III | epican | Extracellular matrix receptor III |

Especificaciones y pureza

Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Especies hospedadoras

Rat

Especificidad

CD44

Inmunógeno

Human CD44

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, PBS Only, Validated

Isotipo

Rat IgG2a

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Imágenes

Flow Cytometry: CD44 Rat mAb (Ab094866)

CD44 Rat mAb (Ab094866) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD44 with CD44 Rat mAb (Ab094866) conjugated with biotin (right panel) compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD44 Rat mAb (Ab094866)

CD44 Rat mAb (Ab094866) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD44 (red) with CD44 Rat mAb (Ab094866) conjugated with biotin and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD44 Rat mAb (Ab094866)

CD44 Rat mAb (Ab094866) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood monocytes labelling CD44 with CD44 Rat mAb (Ab094866) conjugated with biotin (right panel) compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD44 Rat mAb (Ab094866)

CD44 Rat mAb (Ab094866) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood monocytes labelling CD44 (red) with CD44 Rat mAb (Ab094866) conjugated with biotin and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• WB:

  Use at an assay dependent concentration.;

• IF/ICC:

  Use at an assay dependent concentration.;

• Flow Cytometry:

  Use at an assay dependent concentration.

• in vivo CD44 blockade in xenografts:

  Use at an assay dependent concentration. ;

• in vitro CD44 blockade:

  Use at an assay dependent concentration. ;

Objetivos asociados (humanos)

CD44 Tbio CD44 antigen (0 Activities)

Discover Target: CD44

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción