GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

IM7

Conjugation

FITC

Descripción general

Rat anti Mouse CD44 Antibody, Monoclonal (IM7), could be used for Flow Cytometry.

Specifications

Product Name

CD44 Rat mAb (FITC) - Primary antibody, specific to CD44

Sinónimos

Ly-24 | Pgp-1 | HERMES | CD44 antigen | CD44 molecule (Indian blood group) | CD44 | CD44R | CDw44 | cell surface glycoprotein CD44 | chondroitin sulfate proteoglycan 8 | CSPG8 | ECMR-III | epican | Extracellular matrix receptor III | GP90 lymphocyte homin

Especificaciones y pureza

ExactAb™, Validated, 0.5 mg/mL

Mecanismos bioquímicos y fisiológicos

Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA

Especies hospedadoras

Rat

Especificidad

CD44

Inmunógeno

Dexamethasone-induced myeloid leukemia M1 cells

Control positivo

Flow Cytometry: C57BL/6 mouse splenocytes

Conjugación

FITC

Grado

ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein G purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: CD44 Rat mAb (FITC) (Ab190174)

CD44 Rat mAb (FITC) (Ab190174) - Flow Cytometry
Flow cytometry analysis of CD44 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD44 Rat mAb (FITC) (Ab190174) at 0.2μg/test for 1 hour at 4°C. Left panel - Rat IgG (FITC) (Ab180311).

Flow Cytometry: CD44 Rat mAb (FITC) (Ab190174)

CD44 Rat mAb (FITC) (Ab190174) - Flow Cytometry
Flow cytometry analysis of CD44 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD44 Rat mAb (FITC) (Ab190174) at 0.2μg/test for 1 hour at 4°C. Blue - Rat IgG (FITC) (Ab180311). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Objetivos asociados (humanos)

CD44 Tbio CD44 antigen (0 Activities)

Discover Target: CD44

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)