CD45R Rat mAb (PE-Cy7) - Primary antibody, specific to CD45R, Rat IgG2a

Cat. No.: Ab191673

GRADE & PURITY Validated, ExactAb™, Ex:565nm, Em:774nm, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Human, Mouse

Isotype

Rat IgG2a

Clone Number

RA3-6B2

Conjugation

PE-Cy7

Descripción general

Rat anti Mouse/Human CD45R Antibody, Monoclonal (RA3-6B2), could be used for Flow.

Specifications

Product Name

CD45R Rat mAb (PE-Cy7) - Primary antibody, specific to CD45R, Rat IgG2a

Sinónimos

LCA | CD45 | T200 | GP180 | IMD105 | B220 | CD45 antigen | CD45R | EC 3.1.3.48 | L-CA | LY5 | protein tyrosine phosphatase, receptor type, C | receptor-type tyrosine-protein phosphatase C | Mouse B220/CD45R PE-Cy7-conjugated Antibody | PE-Cy7 Anti-Mouse C

Especificaciones y pureza

ExactAb™, Validated, Ex:565nm, Em:774nm, 0.5 mg/mL

Mecanismos bioquímicos y fisiológicos

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affec

Especies hospedadoras

Rat

Especificidad

CD45R

Inmunógeno

Abelson murine leukemia virus-induced pre-B tumor cells

Control positivo

Flow: C57BL/6 mouse splenocytes.

Conjugación

PE-Cy7

Excitación (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emisión (nm)

774nm

Ex/Em(nm)

Ex:565nm;Em:774nm

Grado

Em:774nm, Ex:565nm, ExactAb™, Validated

Isotipo

Rat IgG2a

Tipo de cadena ligera

kappa

Método de purificación

Protein A purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: CD45R Rat mAb (PE-Cy7) (Ab191673)

CD45R Rat mAb (PE-Cy7) (Ab191673) - Flow Cytometry
Flow cytometry analysis of CD45R expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD45R Rat mAb (PE-Cy7) (Ab191673) at 0.2μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE-Cy7) (Ab190073).

Flow Cytometry: CD45R Rat mAb (PE-Cy7) (Ab191673)

CD45R Rat mAb (PE-Cy7) (Ab191673) - Flow Cytometry
Flow cytometry analysis of CD45R expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD45R Rat mAb (PE-Cy7) (Ab191673) at 0.2μg/test for 1 hour at 4°C. Blue - Rat IgG (PE-Cy7) (Ab190073). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume ;

Objetivos asociados (humanos)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)

Discover Target: PTPRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción