CD47 Rat mAb - Primary antibody, specific to CD47, Rat IgG2a

Cat. No.: Ab094972

GRADE & PURITY Carrier Free, Validated, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay, IF/ICC

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

MIAP301

Conjugation

Unconjugated

Descripción general

Rat anti Mouse CD47 Antibody, Monoclonal (MIAP301), could be used for Flow, in vivo CD47 blockade, in vitro CD47 blockade, IF, ICC and so on.
Application
Flow: Use at an assay dependent concentration.
in vivo CD47 blockade: Use at an assay dependent concentration.
in vitro CD47 blockade: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent concentration.
Protein Function
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.

Specifications

Product Name

CD47 Rat mAb - Primary antibody, specific to CD47, Rat IgG2a

Sinónimos

Itgp | 9130415E20Rik | B430305P08Rik | antigen identified by monoclonal 1D8 | Antigenic surface determinant protein OA3 | CD47 antigen (Rh-related antigen, integrin-associated signal transducer) | CD47 antigen | CD47 glycoprotein | CD47 molecule | CD47 |

Especificaciones y pureza

Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Especies hospedadoras

Rat

Especificidad

CD47

Inmunógeno

Intact CD47 purified from placenta

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, PBS Only, Validated

Isotipo

Rat IgG2a

Tipo de cadena ligera

kappa

Método de purificación

Protein G purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Imágenes

Flow Cytometry: CD47 Rat mAb (Ab094972)

CD47 Rat mAb (Ab094972) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD47 with CD47 Rat mAb (Ab094972) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD47 Rat mAb (Ab094972)

CD47 Rat mAb (Ab094972) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD47 (red) with CD47 Rat mAb (Ab094972) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• IF/ICC:

  Use at an assay dependent concentration.;

• Flow Cytometry:

  Use at an assay dependent concentration.

• in vivo CD47 blockade:

  Use at an assay dependent concentration.

• in vitro CD47 blockade:

  Use at an assay dependent concentration.

Objetivos asociados (humanos)

CD47 Tchem Leukocyte surface antigen CD47 (0 Activities)

Discover Target: CD47

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción