CD54 Rat mAb (Biotin) - Primary antibody, specific to Icam1, Rat IgG2b

Cat. No.: Ab187181

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

YN1/1.7.4

Conjugation

Biotin

Descripción general

Flow: ≤0.3 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Specifications

Product Name

CD54 Rat mAb (Biotin) - Primary antibody, specific to Icam1, Rat IgG2b

Sinónimos

BB2 | CD54 antigen | CD54 | cell surface glycoprotein P3.58 | human rhinovirus receptor | ICAM1 | ICAM-1 | intercellular adhesion molecule 1 (CD54), human rhinovirus receptor | intercellular adhesion molecule 1 | Major group rhinovirus receptor | P3.58 |

Especificaciones y pureza

ExactAb™, Validated, 0.5 mg/mL

Especies hospedadoras

Rat

Especificidad

Icam1

Inmunógeno

Mouse NS-1 cells

Control positivo

Flow: C57BL/6 mouse splenocytes.

Conjugación

Biotin

Grado

ExactAb™, Validated

Isotipo

Rat IgG2b

Método de purificación

Protein G purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: CD54 Rat mAb (Biotin) (Ab187181)

CD54 Rat mAb (Biotin) (Ab187181) - Flow Cytometry
Flow cytometry analysis of CD54 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD54 Rat mAb (Biotin) (Ab187181) at 0.03μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Objetivos asociados (humanos)

ICAM1 Tchem Intercellular adhesion molecule 1 (0 Activities)

Discover Target: ICAM1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción