CD73 Rat mAb (Biotin) - Primary antibody, specific to CD73

Cat. No.: Ab191810

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

TY/23

Conjugation

Biotin

Descripción general

Rat anti Mouse CD73 Antibody, Monoclonal (TY/23), could be used for Flow.

Specifications

Product Name

CD73 Rat mAb (Biotin) - Primary antibody, specific to CD73

Sinónimos

Nt5 | 2210401F01Rik | 5' nucleotidase (CD73) | 5'-NT | 5-NT | 5'-Nucleotidase | 5'-nucleotidase, ecto (CD73) | CD73 antigen | CD73 | E5NT | EC 3.1.3.5 | ecto-5'-nucleotidase | eN | eNT | NT | NT55'-nucleotidase | NT5E | NTE | Purine 5-Prime-Nucleotidase |

Especificaciones y pureza

ExactAb™, Validated, 0.5 mg/mL

Mecanismos bioquímicos y fisiológicos

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

Especies hospedadoras

Rat

Especificidad

CD73

Inmunógeno

BALB/c mouse spleen cells and CHO cells transfected with the mouse CD73 gene

Control positivo

Flow: C57BL/6 mouse splenocytes

Conjugación

Biotin

Grado

ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein G purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: CD73 Rat mAb (Biotin) (Ab191810)

CD73 Rat mAb (Biotin) (Ab191810) - Flow Cytometry
Flow cytometry analysis of CD73 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD73 Rat mAb (Biotin) (Ab191810) at 0.5 μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG (Biotin) (Ab180310).

Flow Cytometry: CD73 Rat mAb (Biotin) (Ab191810)

CD73 Rat mAb (Biotin) (Ab191810) - Flow Cytometry
Flow cytometry analysis of CD73 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD73 Rat mAb (Biotin) (Ab191810) at 0.5 μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤1.0 μg/test, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume ;

Objetivos asociados (humanos)

NT5E Tchem 5'-nucleotidase (0 Activities)

Discover Target: NT5E

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)