CD83 Consejo mAb - Primary antibody, specific to CD83, Rat IgG1

Cat. No.: Ab095443

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, ELISA, Flow Cytometry, Functional Assay

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG1

Clone Number

Michel-17

Conjugation

Unconjugated

Descripción general

Anticuerpo monoclonal de rata contra CD83 de ratón (Michel-17), se puede utilizar para el acoplamiento in vivo de CD83, bloqueo in vitro de CD83, flujo, ELISA, etc.
Aplicación:
in vivo CD83 engagement: Utilizar a una concentración dependiente del ensayo.
bloqueo in vitro de CD83: Utilizar a una concentración dependiente del ensayo.
Flujo: Utilizar a una concentración dependiente del ensayo.
ELISA: Usar a una concentración dependiente del ensayo.
Función proteica
Puede desempeñar un papel importante en la presentación de antígenos o en las interacciones celulares que siguen a la activación de linfocitos

Specifications

Product Name

CD83 Consejo mAb - Primary antibody, specific to CD83, Rat IgG1

Sinónimos

BL11 | Antígeno BL11CD83 | Antígeno CD83 (linfocitos B activados, superfamilia de inmunoglobulinas) | Molécula CD83 | CD83 | Proteína de superficie celular HB15 | Glicoproteína de superficie celular | HB15 | HB15hCD83 | Activación de células B Glicoproteí

Especificaciones y pureza

Sin portador, ExactAb™, sin azida, validado, ≥95%(SDS-PAGE), Ver COA

Especies hospedadoras

Rat

Especificidad

CD83

Inmunógeno

CD83-Ig fusion proteins

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotipo

Rat IgG1

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

See COA

Almacenamiento y envío

Forma

Líquido

Condiciones de almacenamiento de almacenamiento

Conservar a 2-8°C,Prohibido congelar

Enviado en

Hielo húmedo

Estabilidad y almacenamiento

Se envía a 4°C. Conservar a 4°C a largo plazo (12 meses). No congelar. Tras la recepción, se recomienda realizar alícuotas.

Imágenes

Flow Cytometry: CD83 Rat mAb (Ab095443)

CD83 Rat mAb (Ab095443) - Flow Cytometry
Flow Cytometry analysis of 1 ng/mL LPS stimulated mouse splenocytes cells (72h) labelling CD83 with CD83 Rat mAb (Ab095443) conjugated with biotin (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD83 Rat mAb (Ab095443)

CD83 Rat mAb (Ab095443) - Flow Cytometry
Flow Cytometry analysis of 1 ng/mL LPS stimulated mouse splenocytes cells (72h) labelling CD83 (red) with CD83 Rat mAb (Ab095443) conjugated with biotin at 0.2 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  Use at an assay dependent concentration.

• ELISA:

  Use at an assay dependent concentration.;

• in vivo CD83 engagement:

  Use at an assay dependent concentration.;

• in vitro CD83 blockade:

  Use at an assay dependent concentration.;

Preguntas frecuentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD83 Rat mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.