Cell Permeabilization Buffer (Triton™ X-100) (10×) - BioReagent,sterile-filtered,10×

Cat. No.: F1508364

GRADE & PURITY Sterile-filtered, BioReagent, 10×

Synonyms

Intracellular Staining Permeabilization Wash Buffer (10×) | Permeabilization Buffer | Intracellular Staining Permeabilization Wash Buffer

Storage

Store at 2-8°C

Shipped In

Wet ice

Application

Flow Cytometry

Descripción general

Product Introduction

Cell Permeabilization Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Cell Permeabilization Buffer is used to permeabilize cells following fixation. It is supplied as a 10× solution and should be diluted in deionized water prior to use.

Key Components:

• Phosphate Buffered Saline (PBS)

• Triton™ X-100: 3% (v/v)

• Proclin 300: 0.05% (v/v) (as preservative)

Usage Protocol:

Centrifuge fixed cells and remove supernatant. Dilute Cell Permeabilization Buffer (10×) to 1× in DI water.  Add diluted Cell Permeabilization Buffer directly to fixed cells, using approximately 100 µl per 1 million cells. Incubate for 10 min at room temperature. Cells may be washed with phosphate-buffered saline (PBS) to remove Cell Permeabilization Buffer prior to antibody incubation, but it is not necessary.

Precautions:

1、The Triton™ X-100 detergent can reduce cell pellet adhesion, so care should be taken to retain cells when removing supernatant following centrifugation.

2、It is recommended that the reagent be titrated for optimal performance for each application.

Specifications

Sinónimos

Intracellular Staining Permeabilization Wash Buffer (10×) | Permeabilization Buffer | Intracellular Staining Permeabilization Wash Buffer

Especificaciones y pureza

BioReagent, sterile-filtered, 10×

Estabilidad y almacenamiento

Store at 2-8℃ long term (12 months).

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C

Enviado en

Wet ice

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Grado

BioReagent, Sterile-filtered

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:
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