Chapman Stone Agar Medium - BioReagent

Cat. No.: C1528621

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Descripción general

For isolation and cultivation of Staphylococcus aureus

Specifications

Especificaciones y pureza

BioReagent

Condiciones de almacenamiento de almacenamiento

Room temperature

Enviado en

Normal

Grado

BioReagent

Product Line

biological_products

Application Protocols

The following provides a general laboratory workflow for dehydrated agar media. Item-specific concentrations, pH, and selective/differential performance for Chapman Stone Agar Medium are not specified for this item; please follow the instructions on the product label/CoA.

General plate preparation (literature/general):

• Weigh the label-specified mass of dehydrated medium and add to the corresponding volume of purified water while stirring.
• Heat with frequent swirling until fully dissolved. Avoid localized overheating.
• Sterilize by autoclaving using a validated cycle appropriate for agar media (e.g., gravity or pre-vacuum). Follow institutional biosafety procedures.
• Cool to ~45–50 C in a water bath. If required, aseptically add sterile-filtered supplements indicated by the specific Chapman Stone formulation.
• Pour into sterile Petri dishes (typically 20–25 mL per 90 mm plate; adjust per lab standard). Allow to solidify and dry surface as needed.

Inoculation and incubation (literature/general):

• Streak or spread sample/controls onto plates. Include appropriate positive/negative control strains to verify selectivity and differential responses.
• Incubate inverted plates at the temperature/time suitable for the target organisms (often 35–37 C for 24–48 h for many staphylococcal workflows; verify for your application).
• Record colony morphology and any differential indicators specified for your formulation; proceed with confirmatory testing as needed.

Storage of poured plates: Typically 2–4 weeks at 2–8 C in sealed bags, protected from light and desiccation (literature/general). For this item, specific shelf-life guidance is not specified; refer to CoA/Spec Sheet.

Biological Roles

This product does not have intrinsic biological activity as a single molecule; rather, it provides a defined environment supporting or inhibiting microbial growth for research purposes.

• Functional concept (literature/general): Chapman/Stone-type media combine a nutrient base with selective agents (e.g., elevated salt, dyes, or tellurite in some historical formulations) and differential indicators (e.g., chromogenic or pH indicators) to highlight physiological traits such as salt tolerance, carbohydrate fermentation, or enzyme activity.
• Microbiological context:
  • Supports growth of target Gram-positive cocci while suppressing competing flora to varying degrees depending on the exact formulation.
  • Enables visual discrimination by colony morphology, color change, precipitation/halo formation, or blackening if tellurite is part of the system (formulation-dependent; not specified for this item).
• Research implications: Useful for selective isolation in mixed microbial samples prior to downstream characterization (e.g., biochemical panels, sequencing). The medium itself does not confer identification; confirmatory testing is recommended.

Item-specific biological performance characteristics (selectivity spectrum, typical colony appearance, target organisms): Not specified for this item; refer to CoA/Spec Sheet and product label.

Buffer Applications

This product is a complete culture medium, not a standalone buffering reagent. While prepared agar media usually include buffering components to maintain pH during incubation, this medium is not intended as a buffer system for biochemical assays.

• For buffer needs, use dedicated buffer reagents (e.g., phosphate, Tris) prepared to defined molarity and pH.
• If pH adjustment of the prepared medium is required, consult the product label/CoA; item-specific target pH is not specified for this item.

Green Alternatives

For culture media, “green chemistry” considerations focus on efficient preparation, reduced waste, and energy management rather than solvent replacement.

• Energy efficiency:
  • Batch autoclaving at full loads improves energy and water use per plate compared with small, frequent runs.
  • Use validated minimum sterilization cycles compatible with the medium to avoid overprocessing.
• Waste reduction:
  • Prepare only the volume needed for near-term use to minimize expired plates.
  • Consider reusable glass plates/tubes where biosafety policy permits, reducing single-use plastics.
• Water stewardship:
  • Use appropriate water purity without excessive polishing steps if not required by the application.
• Comparative considerations (literature/general):
  • Dehydrated media prepared in-house typically have a lower shipping footprint than pre-poured plates but require on-site energy for sterilization.

Small comparison (general):

• Dehydrated medium (this item): Lower shipping mass/volume; customizable; requires autoclave energy and labor.
• Ready-to-use plates: Higher shipping volume/cold chain in some cases; reduced on-site energy/labor; consistent plate geometry; shorter shelf life after receipt.

Pharmaceutical Uses

No therapeutic or clinical uses are claimed. For research use only (per Product Data).

• Typical non-clinical contexts (literature/general): Employed in industrial and academic microbiology research, and potentially in non-compendial quality-control studies where selective recovery of salt-tolerant Gram-positive cocci is desired. This does not imply suitability for regulated compendial methods unless validated.
• Regulatory status: Not specified for this item; refer to CoA/Spec Sheet. If use in regulated environments is intended, perform method suitability and growth-promotion testing under your QMS.

Physical Properties

Item-specific physical constants are not applicable for a multi-component dehydrated medium. Where relevant, generalized properties are provided for laboratory planning.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet. (Typical for dehydrated media: free-flowing powder, color varies by formulation — literature.)
• Solubility: Designed to be reconstituted in purified water to the label-specified concentration (literature/general). Exact concentration: Not specified for this item; refer to CoA/Spec Sheet.
• pH (prepared medium): Not specified for this item; refer to CoA/Spec Sheet. (Media usually list a pH at 25 C after sterilization — literature.)
• Gelling agent: Agar (general for agar media; specific inclusion level not specified for this item).
• Density, BP, MP, refractive index, logP, pKa: Not applicable/not defined for a complex solid mixture.

Practical notes (literature/general):

• Hydration improves with gradual addition of powder to vigorously stirred water; localized overheating can cause clumping.
• Agar gels typically solidify near ~40–45 C and melt near ~85–90 C (literature, general for agar). Exact values for this medium are not specified for this item.

Quality and Grades

• Grade provided: BioReagent (per Product Data). This indicates suitability for research and laboratory applications, with controls on bioburden/endotoxin typical of reagents intended for biological workflows (not pharmaceutical/clinical use unless otherwise stated).
• Research Use Only: Explicitly “For research use only” (per Product Data). Not for diagnostic, therapeutic, or clinical applications.
• Item-specific specifications (e.g., composition, pH, fill weight, sterility, microbial limits): Not specified for this item; refer to CoA/Spec Sheet for lot-specific QC values.

Interpreting BioReagent for dehydrated media (general guidance):

• Emphasis on reproducibility of growth/differentiation performance in standard test organisms.
• Typical QC may include pH of prepared medium, gel strength, and growth promotion/inhibition with defined strains (literature/general), but refer to this item’s CoA for actual tests.

Stabilizers/antimicrobials:

• No stabilizers or preservatives are specified for this item in the Product Data. If present in the formulation, they will be listed on the label/CoA. Always verify before use in sensitive assays.

Reaction and Applications

This section focuses on laboratory applications of Chapman Stone Agar Medium in microbiology rather than chemical reactions.

• Intended use (research): Selective/differential plating medium used in microbiological research to recover and distinguish salt-tolerant Gram-positive cocci, particularly staphylococci, depending on the exact Chapman/Stone formulation (literature/general). Exact selectivity for this product is not specified; consult CoA/label.
• Typical research applications (literature/general):
  • Isolation and enumeration of target organisms from environmental, food, cosmetic, or process samples in research settings.
  • Morphological and colorimetric differentiation based on carbohydrate fermentation, tellurite reduction, lecithinase/lipase activity, or salt tolerance (depending on formulation).
  • Strain maintenance as working plates for routine subculture when selectivity is desired.
• Practical tips:
  • Use freshly prepared plates (≤2–4 weeks refrigerated, protected from light, unless otherwise specified on CoA).
  • Dry plates before streaking to avoid swarming or confluent growth.
  • Include positive/negative control strains to verify selectivity/differential characteristics of each lot (growth-promotion testing; literature/general).
• Limitations: Overly rich inocula or stressed cells may overcome or fail selectivity. Confirmatory tests (biochemical/molecular) are recommended for organism identification in research workflows.

Reaction Conditions

Not applicable to chemical reactions. For preparation and use conditions of the culture medium, see Application Protocols. General preparation involves reconstitution in water, heating to dissolve, sterilization by autoclave, and pouring plates at ~45–50 C (literature/general). Item-specific concentrations, pH, and sterilization parameters are not specified for this item; refer to CoA/Spec Sheet.

Safety and Handling

• GHS classification, signal word, pictograms, H-statements: Not specified for this item; refer to the SDS for authoritative hazard information.
• Primary hazards (general): Dehydrated media can produce nuisance dust; avoid inhalation. Some selective agents used in staphylococcal media (e.g., tellurite) are hazardous in concentrated form; handle the formulated powder per the SDS for this specific product.
• PPE (general lab): Lab coat, safety glasses, and appropriate gloves. Use dust control (weighing in a fume hood) if dusting occurs.
• Handling:
  • Avoid creating dust; close containers promptly to limit moisture uptake.
  • Prepare with purified water; heat with agitation to dissolve. Avoid scorching.
  • Sterilize by autoclave per your lab’s biosafety procedures.
• Incompatibilities: Store away from strong oxidizers and moisture. Do not mix with disinfectants prior to sterilization.
• First aid (overview; defer to SDS):
  • Inhalation: Move to fresh air; seek medical attention if irritation persists.
  • Skin/eyes: Rinse with water; remove contaminated clothing; seek medical attention if irritation persists.
  • Ingestion: Rinse mouth; do not induce vomiting; seek medical advice.
• Waste & biohazard: Treat spent plates/broths as biohazardous; autoclave before disposal in accordance with institutional and local regulations.

Always consult the product-specific SDS and your institutional biosafety guidelines before use.

Solvent Selection

This product is not used as an organic-phase solvent. It is a dehydrated medium intended to be reconstituted in water.

• Primary solvent: High-purity water (Type II or better is commonly used for culture media; literature/general). Avoid high TOC or ionic contaminants that can alter selectivity.
• Water quality considerations (general):
  • Resistivity ≥ 1 MΩ·cm and low TOC help reproducibility of selective/differential responses.
  • Avoid phosphate carryover if the medium’s buffering relies on other systems.
• Preparation temperature: Heat is typically required to dissolve agar; do not boil vigorously to avoid caramelization of carbohydrates (general media practice). Cool to ~45–50 C before pouring plates (literature/general).

If your workflow requires additives (antibiotics, supplements), sterilize the base medium first and add sterile-filtered supplements after cooling to pouring temperature. Verify compatibility with the specific Chapman Stone formulation used.

Storage and Reconstitution

• Storage conditions (as supplied): Room temperature (per Product Data). Protect from moisture and excessive heat. Keep container tightly closed.
• Shipping: Shipped under normal conditions (per Product Data).
• Reconstitution: Dissolve in purified water at the concentration specified on the product label; heat to dissolve; sterilize by autoclave (literature/general). Item-specific mass-per-liter and pH targets are not specified for this item; refer to CoA/Spec Sheet.
• Plate storage (prepared): Not specified for this item; refer to CoA/Spec Sheet. General practice: store poured plates at 2–8 C, protected from light, and use within a few weeks (literature/general).
• Freeze–thaw: Not applicable for dehydrated powder. Avoid freezing prepared agar plates to prevent cracking and syneresis.

Always consult the lot-specific CoA/Spec Sheet for exact preparation parameters and shelf-life guidance.

Structure and Identity

This product is a complex, dehydrated microbiological culture medium rather than a single defined molecule.

• Product type: Dehydrated culture medium (mixture)
• Common name: Chapman Stone Agar Medium (selective/differential agar base for microbiological research)
• SKU: C1528621
• Grade: BioReagent (per Product Data)
• CAS / CID / InChIKey / SMILES: Not applicable to complex mixtures; not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula / Molecular weight: Not applicable to mixtures; not specified for this item; refer to CoA/Spec Sheet.
• Structural features (description): Being a formulated medium, it comprises multiple components (typical media include peptones/enzymatic digests, selective agents, salts, and indicators) combined with agar as the gelling matrix. No single 2D structure applies.

Notes (literature/general):

• “Chapman-type” selective media historically emphasize high-salt and/or tellurite/egg-yolk systems for differentiation of salt-tolerant staphylococci (literature). Exact composition varies by manufacturer and specific formulation; consult the product label/CoA for definitive composition for this item.

Synthetic Utility

Not applicable. Chapman Stone Agar Medium is not a synthetic organic reagent and is not used for chemical transformations. For guidance on this product’s relevant use, see Reaction & Applications and Application Protocols.

Target Specificity

This section applies to antibodies/biological binders and is not applicable to culture media.

• Item-specific target/epitope/clone/isotype: Not applicable.