Cholesterol Oxidase from microorganism, CAS No.9028-76-6

CAS: 9028-76-6 Cat. No.: C139686 Número EC: 232-842-1

GRADE & PURITY EnzymoPure™, ＞10U/mg

Descripción general

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This Cholesterol oxidase has been used in a study to improve treatment of oxysterol-mediated cytotoxicity. Cholesterol oxidase has also been used in a study that determined that cholesterol depletion impairs coupling between channel opening and vesicle release by allowing voltage-gated calcium channels to move further from release sites. Cholesterol oxidase has been used in a study to improve treatment of oxysterol-mediated cytotoxicity. Cholesterol oxidase has also been used in a study that evaluated the effects of cholesterol depletion. This study reported that cholesterol depletion impairs the coupling between channel opening and vesicle release. Additionally, cholesterol oxidase is used to determine serum cholesterol levels. The enzyme also finds application in the microanalysis of steroids in food samples. It has also been used for distinguishing 3-ketosteroids from 3β-hydroxysteroids. Transgenic plants expressing cholesterol oxidase have been investigated in the fight against the cotton boll weevil. CHOD has also been used as a molecular probe to elucidate cellular membrane structures.
Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. The pH optimum of the enzyme is 7.0 to 7.5 and temperature optimum is 50 °C. The pH stability is 5.7-7.8. Hg2+, Ag+, and ionic detergents inhibit the enzyme activity. Molecular mass of the enzyme is 62 kDa. Pathogenic bacteria require CHOD to infect a host′s macrophage

Specifications

Product Name

Cholesterol Oxidase from microorganism, CAS No.9028-76-6

Sinónimos

Oxidase cholesterol | Cholesterol: oxygen oxidoreductase | Cholesterol Oxidase microbial

Grado

EnzymoPure™

Especificaciones y pureza

EnzymoPure™, ＞10U/mg

Mecanismos bioquímicos y fisiológicos

胆固醇氧化酶及胆固醇脂酶一起用于血脂类项目如高密度脂蛋白（HDL-C）、低密度脂蛋白（LDL-C）以及总胆固醇（CHO）等。

CAS

9028-76-6

Número de la Comisión de Enzimas

1.1.3.6

Tipo de molécula

Enzyme

Almacenamiento y envío

Concentración

＞10U/mg

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

Definición de unidad

One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization

Referencias

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