Coelenterazine 400a - Suitable for molecular biology, ≥95%(TLC) , CAS No.70217-82-2

CAS: 70217-82-2 Cat. No.: C1375502 Peso molecular: 391.46

GRADE & PURITY Suitable for molecular biology, ≥95%(TLC)

Synonyms

8-Dibenzyl-6-phenyl-imidazo[1 | 2a]pyrazin-3-(7H)-one

Storage

Protected from light,Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

Descripción general

Coelenterazine 400a is a derivative of coelenterazine and is a good substrate for Renilla reniformis luciferase (RLuc), but does not oxidize well with Gaussia (GLuc). It is the preferred substrate for BRET (Bioluminescence Resonance Energy Transfer) since its emission maximum of 400nm has minimal interference with the GFP emission.
Note: It is not recommended to store dissolved coelenterazine 400a at -20°C or -70°C. Coelenterazine 400a is a high energy dioxetanone ring structure that will spontaneously decompose even at low temperatures.

Specifications

Sinónimos

8-Dibenzyl-6-phenyl-imidazo[1 | 2a]pyrazin-3-(7H)-one

Especificaciones y pureza

Suitable for molecular biology, ≥95%(TLC)

Condiciones de almacenamiento de almacenamiento

Protected from light, Store at -20°C, Argon charged

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Grado

Suitable for molecular biology

Pureza

≥95%(TLC)

Nombres e identificadores

• Sonrisas canónicas: C1=CC=C(C=C1)CC2=C(N3C=C(N=C(C3=N2)CC4=CC=CC=C4)C5=CC=CC=C5)O
• IUPAC Name: 2,8-dibenzyl-6-phenylimidazo[1,2-a]pyrazin-3-ol
• InChIKey: XNNYOKUWNAAJQW-UHFFFAOYSA-N
• INCHI: 1S/C26H21N3O/c30-26-23(17-20-12-6-2-7-13-20)28-25-22(16-19-10-4-1-5-11-19)27-24(18-29(25)26)21-14-8-3-9-15-21/h1-15,18,30H,16-17H2
• Isómeros SMILES: C1=CC=C(C=C1)CC2=C(N3C=C(N=C(C3=N2)CC4=CC=CC=C4)C5=CC=CC=C5)O
• Peso molecular: 391.46
• Reaxy-Rn: 36019358
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=36019358&ln=

Propiedades químicas y físicas

• Solubilidad: Soluble in methanol or ethanol (NOT in DMSO).
• Índice de refracción: n20D1.66 (Predicted)
• Punto de ebullición (°C): 551.39° C at 760 mmHg (Predicted)
• Punto de fusión (°C): 94-98° C
• Peso molecular: 391.500 g/mol
• XLogP3: 6.200
• Hydrogen Bond Donor Count: 1
• Hydrogen Bond Acceptor Count: 3
• Rotatable Bond Count: 5
• Exact Mass: 391.168 Da
• Monoisotopic Mass: 391.168 Da
• Topological Polar Surface Area: 50.400 Ų
• Heavy Atom Count: 30
• Formal Charge: 0
• Complexity: 524.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

Referencias

1. Liu Wang, Yangyi Liu, Jing Sun, Juanjuan Su, Jing Feng, Li Miao, Weijie Zhao, Hongjie Zhang, Kai Liu.  (2025)  In Situ Ultrasound-Triggered Bioluminescence for Combined Sono/Photodynamic Immunotherapy.  ACS Nano,      [PMID:40671351] [10.1021/acsnano.5c06999]