BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C,Protected from light,Room temperature Ships Wet ice Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Copper is an essential trace element in the human body and a key component of enzymes such as ceruloplasmin (ferroxidase), superoxide dismutase, and cytochrome oxidase. The total body copper content is approximately 90 mg, with 90–95% bound to globulin as ceruloplasmin and 5–10% bound to albumin, which serves as a plasma copper carrier. Detection methods for copper include spectrophotometry, atomic absorption spectroscopy, and neutron activation analysis.
Detection Principle
In an acidic medium, copper bound to albumin dissociates. After protein precipitation, copper ions chelate with Cuprizone to form a stable blue complex. The absorbance is measured at 620 nm using a spectrophotometer. The intensity of the blue color is linearly related to the copper concentration in serum or plasma. By comparing the sample absorbance to a standard, the copper content is calculated using a formula. This assay kit demonstrates good linearity up to 62.8 μmol/L, high specificity, and considerable sensitivity. This kit is intended for research use only and is not suitable for clinical diagnosis or other purposes.
| C1507287 | Component | 50T | Storage |
| C1507287A | Copper Standard (100 μg/mL) | 1 mL | 2-8℃ |
| C1507287B | Copper Standard Diluent | 10 mL | RT |
| C1507287C | Cu Acidification Solution | 20 mL | RT |
| C1507287D | Lea Protein Precipitation Solution | 30 mL | RT. Store in the dark. |
| C1507287E | Cu Assay Buffer | 75 mL | 2-8℃ |
| C1507287F | Cuprizone Chromogenic Solution | 5.5 mL | 2-8℃. Store in the dark. |
| C1507287G | ddH₂O | 10 mL | RT |
Materials Required but Not Provided
Centrifuge tubes or test tubes, Centrifuge, Spectrophotometer, Cuvettes
Experimental Procedure
1. Sample Preparation
Plasma or serum samples prepared by standard methods can be used directly. Store at -20°C for Cu detection.
2. Preparation of Copper Standard Working Solution
Dilute the Copper Standard (100 μg/mL) with Copper Standard Diluent at a ratio of 1:24 (Copper Standard : Diluent) to obtain a Copper Standard Working Solution (4 μg/mL). Store at 4°C protected from light; stable for 3 months.
3. Cu Assay Setup
It is preferable to use dry test tubes cleaned with dilute hydrochloric acid and deionized water, or disposable sterile polyethylene centrifuge tubes. If these conditions cannot be met, strictly avoid using utensils contaminated with copper. Proceed according to the table below.
| Reagent (mL) | Blank Tube | Standard Tube | Test Tube |
| ddH₂O | 0.5 | — | — |
| Copper Standard (4 μg/mL) | — | 0.5 | — |
| Test Sample | — | — | 0.5 |
| Cu Acidification Solution | 0.35 | 0.35 | 0.35 |
| Mix thoroughly and incubate at room temperature for 10 min. |
| Lea Protein Precipitation Solution | 0.5 | 0.5 | 0.5 |
Mix thoroughly, incubate at room temperature for 10 min, then centrifuge at 3000 × g for 10 min. Collect the supernatant from each tube.
| Resulting Supernatant | 1 | 1 | 1 |
| Cu Assay Buffer | 1.4 | 1.4 | 1.4 |
| Cuprizone Chromogenic Solution | 0.1 | 0.1 | 0.1 |
4. Cu Measurement
Mix well and incubate at room temperature for 20 minutes. Using the Blank tube to zero the instrument, measure the absorbance of the Standard and Test tubes at 620 nm using a spectrophotometer (recorded as Astandard and Atest ).
5. Calculation of Results
Plasma/Serum Copper (μmol/L) = (Atest / Astandard ) × 62.8
Parameter Description:
Atest : Absorbance of the test well
Astandard : Absorbance of the standard well
Copper Standard (4 μg/mL) is equivalent to 62.8 μmol/L
Unit Conversion: μg/dL = μmol/L / 0.157
Reference Interval:
| Sample Type | Serum Copper Concentration |
| Healthy Adult Males | 10.99 ~ 21.98 μmol/L (70 ~ 140 μg/dL) |
| Healthy Adult Females | 12.56 ~ 23.55 μmol/L (80 ~ 150 μg/dL) |
Precautions
If the sample concentration is too high, dilute with deionized water and re-assay, multiplying the result by the dilution factor.
Use high-purity deionized water throughout the procedure, not ordinary distilled water.
Glassware should ideally be soaked in 10% hydrochloric acid for 24 hours and rinsed thoroughly with deionized water before use.
Avoid copper contamination from instruments, test tubes, syringes, etc., during the experiment.
The measurement wavelength can be selected between 600–640 nm with minimal impact on results.
The color developed by this kit is stable and can be maintained for 1 hour at 4–20°C.
Color may fade or disappear over time; complete measurements within 1 hour.
Store Cu Assay Buffer sealed tightly immediately after use to prevent evaporation of active components.
The classical standard uses a Copper Standard (2 μg/mL). If instrument precision is high and interference is low, this standard can be used, and the calculation formula becomes: Plasma/Serum Copper (μmol/L) = (Atest / Astandard ) × 31.4.
Use reagents promptly after opening to prevent potential effects on subsequent experiments.
Appendix
Reference Standard Curve Range: (Zeroed with Blank)
Absorbance (OD) for the 2 μg/mL Copper Standard typically ranges from 0.05 to 0.07 when measured by spectrophotometer.
Absorbance (OD) for the 4 μg/mL Copper Standard typically ranges from 0.10 to 0.13.
The standard curve obtained by measuring the absorbance of copper standards at 0.1, 0.5, 1, 2, 4, 8, and 16 μg/mL is shown below:
Note: Reference ranges may vary due to differences in detection instruments and operational techniques. These values are for reference only. For precise copper quantification, multi-point calibration using a standard curve is recommended. Empirical data suggests deviations in the standard curve may occur for standard concentrations below 0.5 μg/mL or above 50 μg/mL.
| C1507287 | Component | 50T | Storage |
| C1507287A | Copper Standard (100 μg/mL) | 1 mL | 2-8℃ |
| C1507287B | Copper Standard Diluent | 10 mL | RT |
| C1507287C | Cu Acidification Solution | 20 mL | RT |
| C1507287D | Lea Protein Precipitation Solution | 30 mL | RT. Store in the dark. |
| C1507287E | Cu Assay Buffer | 75 mL | 2-8℃ |
| C1507287F | Cuprizone Chromogenic Solution | 5.5 mL | 2-8℃. Store in the dark. |
| C1507287G | ddH₂O | 10 mL | RT |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View BioReagent grade guide →
