DAF-2 (DMSO solution) - 5 mM in DMSO , CAS No.205391-01-1

CAS: 205391-01-1 Cat. No.: D276597 Peso molecular: 362.34 Número EC: 634-985-9

GRADE & PURITY 5 mM in DMSO

Synonyms

4,5-Diaminofluorescein | 2-(3,6-Dihydroxy-9H-xanthen-9-yl)-4,5-diamino-benzoic acid | DTXSID60443252 | AS-78359 | 5,6-Diamino-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one | DAF-2-CAS 205391-01-1-Calbiochem | Spiro[isobenzofuran-1(3H),9'-[9H]

Storage

Protected from light,Desiccated,Store at -80°C

Shipped In

Dry ice packs + Cold packs

Descripción general

Store at -20°C. Store In the Dark. Store under desiccating conditions. This product is air and light sensitive and impurities can occur as a result of air oxidation or due to metabolism by microbes.

description：

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein compound (DAF-2T). Can detect NO under neutral conditions (detection limit = 5 nM).

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vivo. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent compound triazolofluorescein (DAF-2 T). Can detect NO under neutral conditions (detection limit = 5 nM). Excitation maximum: 495 nm; emission maximum: 515 nm.

Specifications

Sinónimos

4, 5-Diaminofluorescein | 2-(3, 6-Dihydroxy-9H-xanthen-9-yl)-4, 5-diamino-benzoic acid | DTXSID60443252 | AS-78359 | 5, 6-Diamino-3', 6'-dihydroxy-3H-spiro[isobenzofuran-1, 9'-xanthen]-3-one | DAF-2-CAS 205391-01-1-Calbiochem | Spiro[isobenzofuran-1(3H), 9'-[9H]

Especificaciones y pureza

5 mM in DMSO

Mecanismos bioquímicos y fisiológicos

Fluorescent nitric oxide probe. DAF-2 solution. Reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein DAF-2T . Determines nitric oxide synthase activity. Membrance impermeable DAF-2 DA ( ab14；Fluorescent indicator fo

Condiciones de almacenamiento de almacenamiento

Protected from light, Desiccated, Store at -80°C

Enviado en

Dry ice packs + Cold packs

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Nota

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Nombres e identificadores

• Sonrisas canónicas: C1=CC2=C(C=C1O)OC3=C(C24C5=CC(=C(C=C5C(=O)O4)N)N)C=CC(=C3)O
• IUPAC Name: 5,6-diamino-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
• InChIKey: LTYUPYUWXRTNFQ-UHFFFAOYSA-N
• INCHI: 1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2
• Isómeros SMILES: C1=CC2=C(C=C1O)OC3=C(C24C5=CC(=C(C=C5C(=O)O4)N)N)C=CC(=C3)O
• Peso molecular: 362.34
• Reaxy-Rn: 8014486
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=8014486&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Clase Benzopyrans
      • Subclass 1-benzopyrans
        • Level5 Dibenzopyrans
          • Level6 Xanthenes

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Clase: Benzopyrans
• Subclass: 1-benzopyrans
• Intermediate Tree Nodes: Dibenzopyrans
• Direct Parent: Xanthenes
• Alternative Parents: Diarylethers Phthalides Benzofuranones Isobenzofurans 1-hydroxy-2-unsubstituted benzenoids Lactones Carboxylic acid esters Amino acids and derivatives Oxacyclic compounds Primary amines Organic oxides Hydrocarbon derivatives
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Xanthene - Diaryl ether - Benzofuranone - Phthalide - Isobenzofuranone - Isocoumaran - Isobenzofuran - Phenol - 1-hydroxy-2-unsubstituted benzenoid - Benzenoid - Carboxylic acid ester - Lactone - Amino acid or derivatives - Ether - Carboxylic acid derivative - Oxacycle - Hydrocarbon derivative - Organooxygen compound - Organonitrogen compound - Organic oxide - Organic nitrogen compound - Organic oxygen compound - Amine - Primary amine - Aromatic heteropolycyclic compound
• Descripción: This compound belongs to the class of organic compounds known as xanthenes. These are polycyclic aromatic compounds containing a xanthene moiety, which consists of two benzene rings joined to each other by a pyran ring.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Propiedades químicas y físicas

• Solubilidad: Supplied in DMSO (5 mM)
• Sensibilidad: air and light sensitive
• Peso molecular: 362.300 g/mol
• XLogP3: 2.100
• Hydrogen Bond Donor Count: 4
• Hydrogen Bond Acceptor Count: 7
• Rotatable Bond Count: 0
• Exact Mass: 362.09 Da
• Monoisotopic Mass: 362.09 Da
• Topological Polar Surface Area: 128.000 Ų
• Heavy Atom Count: 27
• Formal Charge: 0
• Complexity: 589.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

Referencias

1. Shuang-Shuang Wan, Jin-Yue Zeng, Han Cheng, Xian-Zheng Zhang.  (2018)  ROS-induced NO generation for gas therapy and sensitizing photodynamic therapy of tumor.  BIOMATERIALS,      [PMID:30218836] [10.1016/j.biomaterials.2018.09.004]