Dual Color SDS-PAGE Protein Sample Loading Buffer

Cat. No.: D752143

GRADE & PURITY 6X, Odorless

Descripción general

Aladdin's Dual Color SDS-PAGE Protein Sample Loading Buffer (6X, Odorless) is an improved protein loading buffer with red and blue dyes, which can be used for the preparation and loading of protein samples onto a gel for SDS-PAGE analysis.This product is safe to use. Instead of odoriferous DTT or 2-Mercaptoethanol, this product employs an odorless and more stable reducing agent with similar reducing capability.In conventional SDS-PAGE, the blue dye migrates exactly the same as bromophenol blue, and the red dye migrates similarly to the blue dye. In Tris-Gly electrophoresis system, the red dye migrates slightly faster than the blue dye in SDS-PAGE gel lower than 15%. While in SDS-PAGE higher than 15%, the red dye migrates slightly slower than the blue dye.This product can be used for lane tracing after membrane transfer. It contains a special red dye which can be transferred to a PVDF or NC membrane to indicate the position of the lane, facilitating operations such as film cutting. Please refer to Figure 1 for the electrophoresis and the transfer results of this product.Figure 1. Aladdin's Dual Color SDS-PAGE Protein Sample Loading Buffer (6X, Odorless) in the UltraBio™ Plus PAGE Precast Gel (Tris-Gly, 12%, 10 wells) after electrophoresis and transfer, as well as in the PVDF membrane after transfer (Blot).

Precautions：

For denaturing PAGE analysis, protein samples mixed with this product is recommended to be heated at 95℃ for 5 minutes. Too high a temperature (e.g., 100℃) or too long a time (e.g., more than 15 minutes) may result in protein degradation or abnormal color change of the indicator.This product must be completely dissolved and mixed well before use.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1. Thaw this product at room temperature or in a water bath no more than 37℃. Place at room temperature immediately once dissolved and store at -20℃ after use.2. Add 1μl of this product per 5μl of protein sample, and mix well. Note: After dilution to 1X, this product can also be used for sample lysis directly.3. Heat at 95℃ for 5-10 minutes to fully denature proteins.Note: If the starting material is large and the genomic DNA content is high, the mixture may be very sticky after boiling. In this case, heat for another 5-10 minutes directly or after addition of more 1X sample loading buffer. The thorough heating will release proteins bound to genomic DNA and break genomic DNA so that the mixture will be not too sticky for sample loading. Appropriate ultrasound or repeated aspiration with a 1ml pipette can also help.4. After cooling to room temperature, centrifuge briefly at room temperature, then load the supernatant onto a SDS-PAGE gel.5. Stop electrophoresis when the red or blue dye reaches the bottom of the gel.

Specifications

Product Name

Dual Color SDS-PAGE Protein Sample Loading Buffer

Especificaciones y pureza

6X, Odorless

Tipo de molécula

Bioreagents/Buffer

Almacenamiento y envío

Concentración

6X, Odorless

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Stored at -20 ℃, valid for at least one year.