DL 633 NHS Ester , CAS No.D638920

CAS: D638920 Cat. No.: D638920

Storage

Store at -20°C,Desiccated

Shipped In

Ice chest + Ice pads

Descripción general

DL 633 Amine-Reactive Dye is an NHS ester-activated derivative of high-performance DL 633 used to fluorescently label antibodies and other proteins that are then used as molecular probes for cellular imaging and other fluorescence detection methods.

Features of DL 633 NHS Ester:

• Specific—NHS ester-activated dye labels proteins and other molecules at primary amines (-NH2)
• Optimized procedure—following the standard protocol results in antibodies with excellent dye:protein ratios and recovery rates for optimum activity and fluorescence labeling

Applications:
• Primary antibody labeling for immunofluorescence microscopy, immunohistochemistry (IHC), Western blotting or ELISA assay
• Target protein labeling for in vitro and in vivo fluorescent detection strategies

DL 633 Amine-Reactive Dye is activated with an N-hydroxysuccinimide (NHS) ester moiety to react with exposed N-terminal α-amino groups or the ε-amino groups of lysine residues to form stable amide bonds. Learn more aboutNHS ester chemistry.

Typical labeling reactions require DL 633 Amine-Reactive Dye to first be dissolved in anhydrous dimethyl formamide (DMF) or another suitable organic solvent before adding a specific molar amount of dye to an amine-free buffer containing the protein to be labeled. However, the high solubility of DL Fluors permits protein solutions to be added directly to specific amounts of the labeling reagent. This feature allows DL 633 Amine-Reactive Dye to be provided in multiple formats with flexible protocols to achieve efficient degrees of labeling.

We also offer Standard and Microscale DL 633 Antibody Labeling Kits for fast and efficient fluorescent labeling of antibodies for use in fluorescence methods. The standard size kit contains all necessary components to perform three separate labeling reactions using 1 mg of IgG or similar quantities of other proteins. The microscale kit contains all of the necessary components to perform five separate labeling reactions using 100 µg of IgG. Both kit sizes include the Amine-Reactive DL 633 NHS-ester in convenient single-use vials as well as purification resin and spin columns for the preparation of ready-to-use conjugate.

• Labeling Target:Proteins

Specifications

Condiciones de almacenamiento de almacenamiento

Store at -20°C, Desiccated

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Propiedades del producto

• Reactividad: Amine

Propiedades químicas y físicas

• Solubilidad: DMF(Dimethylformamide)