ELISA Substrates for alkaline phosphatase (AP), CAS No.E491262

CAS: E491262 Cat. No.: E491262

GRADE & PURITY medium

Descripción general

Product usage advice

Incubation temperature: 20-37 °C / 68-100 °F

Response time: Moderate (up to 30 minutes) at 20-37°C

Shelf life: 24 months

Active ingredients and principle of action

In different buffer solutions (pH = 9.5), different additives were added as required, and the active ingredient p-nitrophenyl phosphate (pNPP) was dissolved.

Alkaline phosphatase transfers phosphate residues to acceptors. Under alkaline conditions, a yellow color will appear due to the formation of nitrophenols.

Main advantage

High absorbance yield comparable to freshly dissolved pNPP tablets

Wide measuring range

low background signal

Low blank drift during long-term storage (<0.18 AU over 24 months)

High color stability after quenching the reaction with used stop solution

Storage, Shipping and Filling Information

Store at 2-8°C protected from light. Under these conditions, the expiration date printed on the label is valid for unopened bottles.

Spontaneous decay increases the background. The rate of decay increases if stored at room temperature. Therefore, storage and transport at room temperature should be avoided. However, the activity of the solution was not affected by storage at room temperature. The solution remains valid beyond the due date, but some applications, especially those that include visual assessments, may be hindered by increased background.

Contaminated or leaked substrate solutions from damaged bottles should not be reused and must be destroyed.

Ship in an isolated container with some refrigerated bags.

The following bottling instructions should be considered:

Work in a dust-free and dark room.

Keep the solution as cool as possible.

Avoid contact of solution with any metal parts.

Thoroughly clean all instruments and containers.

Wear powder-free gloves when bottling.

Close the bottle immediately to minimize the effects of light and dust.

Use an opaque, clean bottle made of HDPE or PP.

General instructions for use in ELISA

Only qualified laboratory staff familiar with the basics of immunological methods should use these solutions.

Substrate solutions can be used in qualitative and quantitative ELISA procedures.

When using a 96-well microtiter plate, it is recommended to add 100 μL of pure substrate per well after incubation and washing. After substrate incubation, the reaction can be stopped and photometric measurements can be performed. Using a higher incubation temperature (37°C) may shorten the incubation time.

stop 2. Further increases in signal cannot be safely ruled out using other commercially available stopping solutions. The addition of stop solution did not change the general shape of the spectrum.

Unstopped and stopped solutions should be measured at 405 nm, background corrected: should be measured at 620 nm.

Waste

Disposal of waste must comply with national and local laws and regulations. Disposal of packaging must comply with national and local disposal regulations.

Specifications

Product Name

ELISA Substrates for alkaline phosphatase (AP), CAS No.E491262

Especificaciones y pureza

medium

CAS

E491262

Tipo de molécula

Enzyme

Almacenamiento y envío

Concentración

medium

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light

Enviado en

Wet ice