Technical articles

108 common laboratory cell lines

No.

Cell line

Species

Cell type

Growth pattern

Commonly used media

Commonly used serum

1

HEK293

Human

Fetal kidney epithelial-like cells

Adherent, epithelial-like

MEM + NEAA

10% FBS

2

HepG2.2.15

Human

Hepatocellular carcinoma cells

Adherent, epithelial-like

DMEM

15% FBS

3

12TAg

Mouse

Pluripotent embryonic stem cell-like

Embryonic-type colonies

DMEM

10% FBS

4

293/CHE-Fc

Human

Kidney epithelial-like cells

Adherent, epithelial-like

DMEM

10% FBS

5

3T3-L1

Mouse

Embryonic fibroblasts / preadipocytes

Adherent, fibroblast-like

DMEM

10% FBS

6

3T6

Mouse

Embryonic fibroblasts

Adherent, fibroblast-like

DMEM

10% FBS

7

A431

Human

Epidermoid squamous cell carcinoma

Adherent, epithelial-like

DMEM

10% FBS

8

A549

Human

Lung carcinoma epithelial cells

Adherent, epithelial-like

F-12 (Kaighn’s)

10% FBS

9

A9

Mouse

Connective tissue fibroblasts

Adherent, fibroblast-like

DMEM

10% FBS

10

AR42J

Rat

Pancreatic tumor epithelial-like cells

Adherent, epithelial-like

Ham’s F-12K

20% FBS

11

AtT-20

Mouse

Pituitary tumor cells

Adherent, epithelial-like

F-10

15% HS + 2.5% FBS

12

B95-8

Marmoset

EBV-infected lymphoid-like cells

Suspension, lymphoid-like

RPMI-1640

10% FBS

13

BALB/3T3

Mouse

Embryonic fibroblasts

Adherent, fibroblast-like

DMEM

10% FBS

14

Bel-7402

Human

Hepatocellular carcinoma cells

Adherent, epithelial-like

RPMI-1640

15% FBS

15

BeWo

Human

Choriocarcinoma epithelial cells

Adherent, epithelial-like

F-12K

15% FBS

16

BHK-21

Hamster

Kidney fibroblast-like cells

Adherent, fibroblast-like

MEM + NEAA

10% FBS

17

HBL-100

Human

Mammary epithelial cells

Adherent, epithelial-like

McCoy’s 5A

10% FBS

18

BRL-3A

Rat

Liver epithelial cells

Adherent, epithelial-like

MEM + NEAA

10% FBS

19

BT

Bovine

Turbinate bone–derived fibroblasts

Adherent, fibroblast-like

DMEM

10% FBS

20

Caco-2

Human

Colonic adenocarcinoma epithelial cells

Adherent, epithelial-like

MEM + NEAA

10% FBS

21

Chang

Human

Liver epithelial cells

Adherent, epithelial-like

BME

10% FCS

22

CHO-K1

Hamster

Ovarian epithelial cells

Adherent, epithelial-like

F-12

10% FBS

23

CL2

Mouse

B cells (hybridoma-like)

Suspension, lymphocyte-like

RPMI-1640

10% FBS

24

CL3

Mouse

B cells (hybridoma-like)

Suspension, lymphocyte-like

RPMI-1640

10% FBS

25

Clone 9

Rat

Liver epithelial cells

Adherent, epithelial-like

F-12K

10% FBS

26

Clone M-3

Mouse

Melanoma cells

Adherent, epithelial-like

F-12

15% HS + 2.5% FBS

27

COS-1

African green monkey

Kidney fibroblast-like cells

Adherent, fibroblast-like

DMEM

10% FBS

28

COS-3

Monkey

Kidney fibroblast-like cells

Adherent, fibroblast-like

MEM

10% FBS

29

COS-7

African green monkey

Kidney fibroblast-like cells

Adherent, fibroblast-like

DMEM

10% FBS

30

CRFK

Cat

Kidney epithelial cells

Adherent, epithelial-like

MEM + NEAA

10% FBS

31

CV-1

African green monkey

Kidney fibroblast-like cells

Adherent, fibroblast-like

MEM + NEAA

10% FBS

32

D-17

Dog

Osteosarcoma cells

Adherent, epithelial-like

MEM + NEAA

10% FBS

33

DAN

Dog

Osteosarcoma cells

Adherent, epithelial-like

MEM/EBSS

8% FBS

34

Daudi

Human

Burkitt lymphoma B cells

Suspension, lymphoid-like

RPMI-1640

10% FBS

35

DLD-1

Human

Colorectal adenocarcinoma cells

Adherent, epithelial-like

RPMI-1640

10% FBS

36

DSDh

Dog

Osteosarcoma cells

Adherent, epithelial-like

MEM/EBSS

8% FBS

37

DSN

Dog

Osteosarcoma cells

Adherent, epithelial-like

MEM + NEAA

10% FBS

38

Du145

Human

Prostate carcinoma cells

Adherent, epithelial-like

MEM + NEAA

10% FBS

39

EB-3

Human

Burkitt lymphoma B cells

Suspension, lymphoid-like

RPMI-1640

10% FBS

40

ES-D3

Mouse

Pluripotent embryonic stem cells

Embryonic-type colonies

DMEM

15% FBS

41

F9

Mouse

Testicular teratocarcinoma embryonal-like cells

Embryonic to endoderm-like

DMEM

15% FBS

42

GH1

Rat

Pituitary tumor cells

Adherent, epithelial-like

F-12

15% HS + 2.5% FBS

43

GH3

Rat

Pituitary tumor cells

Adherent, epithelial-like

F-12

15% HS + 2.5% FBS

44

H9

Human

T-cell lymphoma cells

Suspension, lymphoblast-like

RPMI-1640

10% FBS

45

H9/HTLV-IIIB

Human

T-cell lymphoma (HIV/HTLV-infected)

Suspension, lymphoblast-like

RPMI-1640

10% FBS

46

HaK

Hamster

Kidney epithelial cells

Adherent, epithelial-like

BME

10% FCS

47

HCT-15

Human

Colorectal adenocarcinoma cells

Adherent, epithelial-like

RPMI-1640

10% FBS

48

HeLa

Human

Cervical carcinoma epithelial cells

Adherent, epithelial-like

MEM + NEAA

10% FBS

49

HeLa S3

Human

Cervical carcinoma subclone

Adherent, epithelial-like

F-12

10% FBS

50

Hep-2

Human

Laryngeal carcinoma epithelial cells (HeLa-like)

Adherent, epithelial-like

MEM

10% FBS

51

Hep3B

Human

Hepatocellular carcinoma cells

Adherent, epithelial-like

MEM + NEAA

10% FBS

52

HepG2

Human

Hepatocellular carcinoma cells

Adherent, epithelial-like

MEM + NEAA

10% FBS

53

HK-2

Human

Renal cortical epithelial cells

Adherent, epithelial-like

Keratinocyte SFM

Serum-free (with rhEGF etc.)

54

HL-60

Human

Acute promyelocytic leukemia cells

Suspension, granulocyte-like

RPMI-1640

20% FBS

55

HP1 (HT1080 poly)

Human

Fibrosarcoma cells

Adherent, epithelial-like

DMEM

10% FBS

56

HT-1080

Human

Fibrosarcoma cells

Adherent, epithelial-like

MEM + NEAA

10% FBS

57

HT-29

Human

Colon adenocarcinoma cells

Adherent, epithelial-like

DMEM/F-12 (1:1)

5% FBS

58

HTC

Rat

Liver epithelial cells

Adherent, epithelial-like

MEM/EBSS

10% FBS

59

HuT 78

Human

T-cell lymphoma cells

Suspension, lymphoblast-like

IMDM

20% FBS

60

HUVEC

Human

Umbilical vein endothelial cells

Adherent, epithelial-like

F-12K

10% FBS + heparin 100 μg/mL

61

HX (HT108 xeno)

Human

Fibrosarcoma (HT1080 xenograft–derived)

Adherent, epithelial-like

DMEM

10% FBS

62

I-10

Mouse

Testicular tumor cells

Adherent, epithelial-like

F-10

15% HS + 2.5% FBS

63

IEC-6

Rat

Normal small intestinal epithelial-like cells

Adherent, epithelial-like

DMEM

5% FBS + insulin 0.1 μg/mL

64

IM-9

Human

Bone marrow B cells from multiple myeloma patient

Suspension, lymphoid-like

RPMI-1640

10% FBS

65

IMR-90

Human

Lung fibroblasts

Adherent, fibroblast-like

MEM + NEAA

10% FBS

66

JAA-F11

Mouse

B-cell hybridoma

Suspension, lymphoid-like

DMEM + NEAA

10% FBS

67

JEG-3

Human

Choriocarcinoma epithelial cells

Adherent, epithelial-like

MEM + NEAA

10% FBS

68

Jensen

Rat

Sarcoma fibroblast-like cells

Adherent, fibroblast-like

McCoy’s 5A

5% FBS

69

Jurkat

Human

Acute T-cell leukemia cells

Suspension, lymphoid-like

RPMI-1640

10% FBS

70

K-562

Human

Chronic myelogenous leukemia cells

Suspension, lymphoblast-like

RPMI-1640

10% FBS

71

KB

Human

Oral carcinoma epithelial cells

Adherent, epithelial-like

MEM + NEAA

10% FBS

72

KG-1

Human

Erythroleukemia bone marrow cells

Suspension, myeloblast-like

IMDM

20% FBS

73

KG-1a

Human

KG-1 subline bone marrow cells

Suspension, myeloblast-like

IMDM

20% FBS

74

L2

Rat

Lung epithelial cells

Adherent, epithelial-like

F-12K

10% FBS

75

L6

Rat

Skeletal muscle myoblasts

Adherent, myoblast-like

DMEM

10% FBS

76

LLC-WRC 256

Rat

Carcinoma cells (Walker 256–derived)

Adherent, epithelial-like

199 medium

5% HS

77

LnCap

Human

Prostate carcinoma cells

Adherent, epithelial-like

RPMI-1640

10% FBS

78

LO2

Human

Normal liver epithelial cells

Adherent, epithelial-like

DMEM

15% FBS

79

M. dunni (Clone IIIBC)

Mouse

Skin fibroblasts

Adherent, fibroblast-like

McCoy’s 5A

10% FBS

80

MARC S5

Mouse

B-cell hybridoma

Suspension, lymphoid-like

MEM + NEAA or DMEM

20% heat-inactivated FBS

81

MCF-7

Human

Breast carcinoma epithelial cells

Adherent, epithelial-like

MEM + NEAA

10% FBS + insulin 10 μg/mL

82

MHCC97

Human

Hepatocellular carcinoma cells

Adherent, epithelial-like

DMEM

15% FBS

83

MOLT-4

Human

Acute T-lymphoblastic leukemia cells

Suspension, lymphoid-like

RPMI-1640

10% FBS

84

NIH3T3

Mouse

Embryonic fibroblasts

Adherent, fibroblast-like

DMEM

15% FBS

85

NK-92

Human

NK-cell lymphoma

Suspension, lymphoblast-like

α-MEM

12.5% HS + 12.5% FBS (+ IL-2)

86

NK-92MI

Human

NK-92 autocrine IL-2 variant

Suspension, lymphoblast-like

α-MEM

12.5% HS + 12.5% FBS

87

PANC-1

Human

Pancreatic carcinoma epithelial cells

Adherent, epithelial-like

DMEM

10% FBS

88

PC12

Rat

Adrenal pheochromocytoma cells

Loosely adherent, easily aggregated

F-12

15% HS + 2.5% FBS

89

PC3

Human

Prostatic adenocarcinoma (bone metastasis)

Adherent, epithelial-like

F-12

10% FBS

90

Raji

Human

Burkitt lymphoma B cells

Suspension, lymphoid-like

RPMI-1640

10% FBS

91

Rat2

Rat

Embryonic fibroblasts (Rat2 clone)

Adherent, fibroblast-like

DMEM

5% FBS + BrdU 30 μg/mL

92

RS4;11

Human

Acute lymphoblastic leukemia cells

Suspension, lymphoblast-like

RPMI-1640

10% FBS

93

Saos-2

Human

Osteosarcoma epithelial-like cells

Adherent, epithelial-like

McCoy’s 5A

15% FBS

94

SMMC7721

Human

Hepatocellular carcinoma cells

Adherent, epithelial-like

RPMI-1640

15% FBS

95

SP2/0

Mouse

Myeloma hybridoma parent line

Suspension, hybridoma-like

IMDM

7.5% FBS

96

SW480

Human

Colon carcinoma epithelial cells

Adherent, epithelial-like

L-15

10% FBS

97

SW620

Human

Colon carcinoma epithelial cells

Adherent, epithelial-like

L-15

10% FBS + 2 mM L-glutamine

98

THP-1

Human

Acute monocytic leukemia cells

Suspension, monocyte-like

RPMI-1640

10% FBS

99

U937

Human

Histiocytic lymphoma / monocytic leukemia cells

Suspension, monocyte-like

RPMI-1640

10% FBS

100

Vero

African green monkey

Kidney epithelial cells

Adherent, epithelial-like

DMEM

10% FBS

101

Vero-E6

African green monkey

Kidney epithelial cells

Adherent, epithelial-like

MEM/EBSS

10% FBS

102

WEHI-3

Mouse

Myelomonocytic leukemia cells

Suspension, macrophage-like

IMDM

10% FBS

103

WI-38

Human

Embryonic lung fibroblast / epithelial-like cells

Adherent, epithelial-like

BME

10% FBS

104

WISH

Human

Amniotic epithelial cells

Adherent, epithelial-like

BME

10% FBS

105

WS1

Human

Embryonic skin epithelial cells

Adherent, epithelial-like

MEM + NEAA

10% FBS

106

WSS-1 (WS-1)

Human

Kidney epithelial cells

Adherent, epithelial-like

DMEM

10% FBS

107

XC

Rat

Sarcoma epithelial-like cells

Adherent, epithelial-like

MEM + NEAA

10% FBS

108

Y-1

Mouse

Adrenal tumor epithelial cells

Adherent, epithelial-like

F-10

15% HS + 2.5% FBS

 

Aladdin: https://www.aladdinsci.com/

Categories: Technical articles
Explore topics: Cell line

Da — when not otherwise indicated, molecular weight units are daltons.   Mw — weight-average molecular weight.   Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "108 common laboratory cell lines" Aladdin Knowledge Base, updated 2 dic 2025. https://www.aladdinsci.com/us_es/faqs/108-common-laboratory-cell-lines-en.html
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