ActiBioPure™: Overview

ActiBioPure™ is the flagship quality designation from Aladdin Scientific, designed for bioactive recombinant proteins and enzymes. It balances high purity, strong biological activity, and batch-to-batch consistency, making it ideally suited for research and industrial applications such as cell signaling studies, functional assays, and therapeutic development.

Key Quality Attributes

· Purity: Typically ≥ 95% (often ≥ 97% by SDS-PAGE and HPLC) depending on the protein.

· Endotoxin Levels: Low—usually < 1.0 EU/μg, suitable for sensitive biological systems.

· Bioactivity: Functionally validated via relevant activity assays—e.g. chemotaxis for CCL1 or proliferation for IL-9, with ED₅₀ values in low ng/mL range.

Typical Applications

Products bearing the ActiBioPure™ label are tailored for:

· Cell-based assays and signal transduction studies

· Enzyme kinetic and biochemical function screens

· Protein engineering and therapeutic candidate evaluation

· In vitro drug metabolism or binding assays

They are not intended for clinical or diagnostic use unless otherwise specified and registered by regulatory authorities.

Product Examples

· Recombinant Human IL-9 Protein (ActiBioPure™)
Purity ≥ 97%, ED₅₀ ≈ 0.5–2 ng/mL in MO7e proliferation assay; endotoxin < 1.0 EU/µg; expressed in insect cells; C-His tag; animal- and carrier-free.

· Recombinant Human CCL1 Protein (ActiBioPure™)
Purity > 97% (SDS-PAGE & HPLC); endotoxin < 1.0 EU/µg; chemotaxis bioassay-based activity 10–100 ng/mL; E. coli expression; animal-free, no tag.

· Enzymes such as Hexokinase or Cytochrome P450 Reductase
Labeled ActiBioPure™ with ≥90% purity, robust enzyme activity (e.g. ≥ 500 U/mg or ≥ 2 U/mL), produced under stringent QA protocols.

Standard Summary Table

Feature	ActiBioPure™ Standard
Purity	≥ 95% (often ≥ 97%)
Endotoxin Level	< 1 EU/µg
Bioactivity	Validated; low- ng/mL ED₅₀ or high U/mg enzyme
Expression Systems	E. coli, insect cells, mammalian depending on product
Intended Use	Research & industrial assays only (not clinical)

Why Choose ActiBioPure™?

· Consistent biofunctional performance across lots

· Minimally contaminated—ideal for sensitive assays

· Simplified formulation with no animal-derived or carrier components

· Supplied with technical documentation: COA, activity, purity, endotoxin data

Additional Notes

· ActiBioPure™ is not a regulatory or pharmacopoeia grade.

· For applications demanding higher purity or clinical-grade compliance, consider additional custom manufacturing steps.

View all  ActiBioPure™ products