AD80 Inhibitor: Key Facts, Uses & FAQs

AD80 is a potent multikinase inhibitor with activity against RET, SRC, S6K, and other oncology-relevant kinases, supplied by Aladdin Scientific as catalog item A275119 for use in preclinical research.

Catalog SKU

A275119

Primary Targets

RET, SRC, S6K (and related kinases)

Compound Class

Multikinase small-molecule inhibitor

Research Application

Oncology signaling pathway research, preclinical cancer models

Recommended Storage

-20 °C, protected from light and moisture (verify with CoA)

Typical Solvent

DMSO (verify concentration limits with CoA/SDS)

What Is AD80 and How Does It Work?

AD80 (Aladdin SKU: A275119) is a multikinase inhibitor designed to simultaneously suppress multiple kinase targets implicated in oncogenic signaling. Its primary targets include RET (rearranged during transfection), SRC family kinases, and S6 kinase (S6K), all of which play roles in tumor cell proliferation, survival, and metastasis.

By binding competitively at or near the ATP-binding pocket of these kinases, AD80 prevents phosphorylation of downstream substrates, thereby interrupting aberrant signaling cascades. The multi-target profile of AD80 may offer advantages in overcoming pathway redundancy compared with single-target agents, making it a valuable tool compound for mechanistic studies in cancer biology.

Solubility, Formulation & Handling Guidelines

AD80 is typically soluble in dimethyl sulfoxide (DMSO) at concentrations suitable for stock solution preparation. Researchers should prepare a concentrated DMSO stock (e.g., 10–50 mM) and dilute into aqueous cell culture media or assay buffers immediately before use. The final DMSO concentration in assay wells should generally be kept at or below 0.1% to minimise cytotoxic artefacts, though optimal limits should be determined empirically for each cell system.

For in vivo formulations, additional solubilisation strategies such as inclusion of PEG400, Tween-80, or cyclodextrin carriers may be required. Always consult the Certificate of Analysis (CoA) and Safety Data Sheet (SDS) provided with the product lot for confirmed solubility data, purity, and handling precautions before commencing experiments.

Important: Avoid repeated freeze-thaw cycles. Aliquot stock solutions into single-use volumes and store at -20 °C.

Recommended Storage Conditions

To maintain the integrity and potency of AD80 over time, the following storage practices are recommended:

• Temperature: Store at -20 °C as a dry powder or in aliquoted solution.
• Light: Protect from direct light exposure; store in amber vials or foil-wrapped containers where possible.
• Moisture: Keep desiccated; allow the container to equilibrate to room temperature before opening to prevent condensation.
• Shelf life: Refer to the expiry date on the product label and CoA. Stability data specific to lot A275119 should be confirmed with Aladdin Scientific technical support.

Improper storage can lead to degradation, reduced biological activity, and inconsistent experimental results. Always verify compound integrity by checking purity (e.g., HPLC or NMR) if long-term storage has occurred before critical experiments.

Research Applications & Experimental Considerations

AD80 is used as a research tool compound in preclinical settings. Documented and plausible research applications include:

• RET-driven cancer models: Investigating RET fusion or mutation-driven oncogenesis in thyroid, lung, and other cancers.
• Combination therapy studies: Assessing synergistic or additive effects when combined with other targeted agents or chemotherapeutics.
• Resistance mechanism studies: Exploring compensatory pathway activation following RET or SRC inhibition.
• Signaling pathway dissection: Using AD80 as a pharmacological probe to delineate RET/SRC/S6K pathway contributions in cellular phenotypes.

Researchers should optimise dose-response relationships for each specific cell line or animal model. Appropriate vehicle controls and negative/positive controls are essential for data interpretation. AD80 is intended for research use only and is not approved for clinical or veterinary use.

Ordering, Purity & Quality Information

AD80 is available from Aladdin Scientific under catalog number A275119. Each lot is accompanied by a Certificate of Analysis (CoA) specifying purity (typically assessed by HPLC), appearance, molecular weight confirmation, and storage recommendations.

To order, visit the Aladdin Scientific website or contact your regional sales representative. For bulk quantities, custom packaging, or specific purity grades, please reach out to technical sales for a quotation. Procurement teams should reference SKU A275119 in all purchase orders to ensure accurate fulfilment.

If you require additional documentation such as Safety Data Sheets (SDS), NMR spectra, or mass spectrometry data, these can be requested from Aladdin Scientific customer support. All quality documents are lot-specific and should be retained for laboratory records and compliance purposes.

Frequently asked questions

What kinase targets does AD80 inhibit?

AD80 is a multikinase inhibitor with documented activity against RET, SRC family kinases, and S6 kinase (S6K), among potentially other related kinases. Its broad kinase inhibition profile makes it useful for studying pathway redundancy and oncogenic signaling networks. Researchers should consult primary literature and the product CoA for confirmed IC50 values for each target.

What is the recommended solvent for dissolving AD80?

AD80 is commonly dissolved in DMSO to prepare concentrated stock solutions. The stock is then diluted into aqueous buffers or cell culture media immediately prior to use, keeping the final DMSO concentration at or below 0.1% to avoid solvent toxicity. Always verify solubility and concentration limits against the lot-specific CoA provided with product A275119.

How should AD80 (A275119) be stored to maintain stability?

AD80 should be stored at -20 °C as a dry powder or in aliquoted DMSO stock solutions, protected from light and moisture. Avoid repeated freeze-thaw cycles by preparing single-use aliquots. Always allow the vial to reach room temperature before opening to prevent moisture condensation. Confirm specific stability data with Aladdin Scientific technical support or the product CoA.

Is AD80 suitable for in vivo studies?

AD80 can potentially be used in preclinical in vivo models, but aqueous solubility limitations may require formulation with co-solvents such as PEG400, Tween-80, or cyclodextrin vehicles. Researchers must determine appropriate dosing, vehicle formulation, and pharmacokinetic parameters for their specific animal model. AD80 is for research use only and is not approved for clinical application.

Where can I find the Certificate of Analysis (CoA) for AD80?

The Certificate of Analysis for AD80 (A275119) can be obtained from the Aladdin Scientific website by entering the product SKU and lot number, or by contacting Aladdin Scientific customer support directly. The CoA contains lot-specific purity data, appearance, and storage information essential for experimental planning.

References

1. Dar AC, et al. Chemical genetic discovery of targets and anti-targets for cancer polypharmacology. Nature. 2012;486(7401):80-84. doi:10.1038/nature11127
2. Aladdin Scientific. Product Page: AD80, SKU A275119. Aladdin Scientific official website.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.