Animal model of spontaneous prostatic hyperplasia

Summary

Dogs, like humans, can develop spontaneous benign prostatic hyperplasia, the incidence of which increases with age, and are recognized as an ideal animal model for human prostatic hyperplasia. There is one major modeling method: the establishment of an animal model of spontaneous prostatic hyperplasia.

Appliance

Ultrasonography of the canine prostate is slightly different from that of humans and must be performed by experienced personnel who have had experience with the procedure and who have had the opportunity to gain experience with it before it can be formalized into an experiment. The volume of the prostate can be measured either by ultrasound or by dissection, which is more difficult but more accurate. This model is the closest to human prostate hyperplasia and is considered to be a suitable animal model for the study of anti-prostatic hyperplasia drugs to date.

Operation method

Animal model of spontaneous prostatic hyperplasia

Principle

The degree of prostatic hyperplasia in dogs correlated with the age of the dog. 1-3 year old dogs had a prostate weight of (14.7±6.4) g, with 25% hyperplastic prostate tissue; 5-10 year old dogs had a prostate weight of (23.6±10.5) g, with 88% hyperplastic prostate tissue. There was no hyperplasia in prostates weighing less than 12.8 g, hyperplasia in those weighing more than 18 g, and a combination of normal and hyperplastic tissue in those weighing 12-18 g. The prostate weights in dogs aged 5-10 years were (23.6±10.5) g and 88% were hyperplastic.

Materials and Instruments

Equipment:
B ultrasound, urinary catheter, bipod swimming ruler, puncture needles, common surgical instruments
Reagents:
① anesthetic
② saline

Move

A. 10-18 kg (7-15 years old) male old dogs, first rectal palpation, found that the prostate gland is obviously enlarged, can then B-type ultrasonography or caesarean section to determine the size of the prostate volume, confirmed that the prostate gland is obviously enlarged for the application of the model.

B. Ultrasonography: Under anesthesia, 200 ml of sterilized saline was injected into the bladder of the dog through a catheter, and the maximal transverse, longitudinal and thick diameters of the prostate were measured accurately with the electronic ruler of the ultrasound instrument, and the three diameters were multiplied together to get the approximation value of the volume of the prostate gland ( ^cm3 ), and those with the volume of the prostate greater than 18 ^cm3 were considered as the qualified models of prostatic hyperplasia.

C. Cesarean section: The prostate gland was exposed through the median incision door of the lower abdomen, and the transverse, head-to-tail and dorso-ventral diameters of the prostate gland were measured with a bipedal measuring tape.

At the time of surgery, the left hemisphere of the gland can be punctured for biopsy and morphologic examination to observe the prostate epithelial cells, papillae, luminal contents, and interglandular connective tissues, and who will measure the height of the epithelial cells and the diameter of the gland lumen by morphometric methods. Pharmacologic studies can be initiated 1 week after surgery.

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