Animal models of nutritional obesity
Animal models of nutritional obesity
Nutritional obesity animal model, also known as food-induced obesity animal model, obesity is an important medical and public health problem facing the world, although the highest rate of growth of childhood obesity exists in the developed countries, but the problem of childhood obesity in developing countries is also increasing year by year. At present, the establishment method of nutritional obesity animal model is mainly the establishment of food-induced obesity animal model.
Principle
Nutritional obesity animal models, i.e., food-induced obesity animal models, are the most desirable animal models of obesity and most closely resemble those produced by humans.
Appliance
Nutritional obesity animal models, i.e., food-induced obesity animal models, are the most desirable animal models of obesity and most closely resemble those produced by humans.
Operation method
Establishment of an animal model of nutritional obesity
Principle
Nutritional obesity animal models, i.e., food-induced obesity animal models, are the most desirable animal models of obesity and most closely resemble those produced by humans.
Materials and Instruments
Experimental animals: weaned Wistar male rats Move The modeling method of nutritional obesity animal model is as follows: A. The rats were fed a high-fat diet or a high-sugar, high-fat diet, and were free to eat and drink, and the temperature of the room was maintained at 20-25 ℃ and the humidity at 40% -60%. C. After 8-16 weeks of high-fat feeding, the body weight exceeds 20% of the body weight of ordinary feed-fed rats, which can be used as experimental obese rats. For more product details, please visit Aladdin Scientific website.
Reagents:
① high-fat feed: ordinary feed 60%, lard 12%, sucrose 5%, milk powder 5%, peanuts 5%, eggs 10%, sesame oil 1%, salt 2%;
② high sugar and high fat feed: 68.8% of the basic feed, 10% sucrose, 10% egg yolk powder, 10% lard, 1% cholesterol, bile salt 0.2%.