Dissolve 60grams per liter of ddH2O, heating to boiling with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15 psi). After cooling to 45-50°C add 10mL of a sterile I% Potassium Tellurite solution and 50mL of sterile egg yolk emulsion.
Component
g/liter
Casein Digest Peptone
10
Lithium Chloride
5
Beef Extract
5
Agar
17
Yeast Extract
1
Glycine
12
Sodium Pyruvate
10
Total: 60g/Liter
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
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