The amount of cells is not 1x10^6, only 1x10^5, can the amount of antibody be reduced?
The amount of cells is not 1x10^6, only 1x10^5, can the amount of antibody be reduced?
The amount of cells is not 1x10^6, only 1x10^5, can the amount of antibody be reduced?
The amount of antibody is related to the incubation system, if the volume of cell suspension is still 100 μL, the amount of antibody is the same, if you want to save the amount of antibody, you can reduce the volume of cell suspension, the amount of antibody can be proportionally reduced. It is recommended to use the recommended amount of cells for the experiment, if the amount of cells is too much, it will lead to insufficient amount of antibody, resulting in false-negative, if the amount of cells is too little, especially when doing the intracellular or intranuclear indexes, a large number of centrifugation will lose a lot of cells, resulting in insufficient number of cells in the final detection.
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