D/E Neutralizing Agar (Powder)

Directions per Liter:

Dissolve 54g/liter in ddH2O with heat. stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi)

Component	g/liter
Yeast Extract	2.5
Dextrose	10
Casein Digest Peptone	5
Lecithin	7
Sodium Thioglycollate	1
Sodium Thiosulfate	6
Sodium Bisulfite	2.5
Polysorbate 80	5
Bromocresol Purple	0.02
Agar	15
	Total: 54g/Liter

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.