Frequently Asked Questions: Products, Experiments and Troubleshooting

Does the protein's own charge affect the buffer?

Does the protein's own charge affect the buffer?

No. The SDS in the buffer binds to the protein and completely covers the protein-SDS complex. The SDS in the buffer binds to the protein and causes the protein-SDS complex to take on a large negative charge, completely covering the protein's own charge.

Categories: Frequently Asked Questions: Products, Experiments and Troubleshooting
Explore topics: Buffer protein Charge

Da — when not otherwise indicated, molecular weight units are daltons.   Mw — weight-average molecular weight.   Mn — number-average molecular weight.

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Aladdin Scientific. "Does the protein's own charge affect the buffer?" Aladdin Knowledge Base, updated 9 jun 2025. https://www.aladdinsci.com/us_es/faqs/does-the-protein-own-charge-affect-the-buffer-en.html
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