Laboratory Protocols & SOPs Library

ELISA Blocking Solution Recipe

· Published 27 jul 2023 · Updated 9 jun 2025

Recipe

1.Dissolve 5.3g of Na2CO3 in 900ml distilled H2O

2.Dissolve 4.2g of NaHCO3 in the solution from step 1

3.Dissolve 1g sodium azide in the solution from step 2

4.Dissolve 5g BSA in solution from step 3

5.Alternatively you can also use 50g non-fat dry milk for blocking solution

6.pH to 9.6

7.Adjust volume to 1L with additional distilled H2O

Categories: Laboratory Protocols & SOPs Library

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "ELISA Blocking Solution Recipe" Aladdin Knowledge Base, updated 9 jun 2025. https://www.aladdinsci.com/us_es/faqs/elisa-blocking-solution-recipe-en.html

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