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ELISA Coating Solution Recipe

Protocols

ELISA Coating Solution Recipe

· Reviewed · Published 9 jun 2025 · Updated 27 jul 2023 · 8 min read

Recipe

1.Dissolve 5.3g of Na2CO3 in 900ml distilled H2O.

2.Dissolve 4.2g of NaHCO3 in the solution from step 1.

3.Dissolve 1g sodium azide in the solution from step 2.

4.pH to 9.6.

5.Adjust volume to 1L with additional distilled H2O.

Categories: Protocols

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "ELISA Coating Solution Recipe" Aladdin Knowledge Base, updated 27 jul 2023. https://www.aladdinsci.com/us_es/faqs/elisa-coating-solution-recipe-en.html

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