Enrichment medium preparation experiment

Summary

Source of content: compact guide to molecular biology 5th ed.

Operation method

Enrichment medium preparation experiment

Principle

Prepare as for Extreme Medium, but at high pressure for 25 min (unless otherwise indicated) and without dilution.

Materials and Instruments

Tryptone NaCl NaOH Glycerol Na2HPO4 KNO3
Glass bottles Flasks

Move

1. In a 2L flask, add the ingredients of the following formula to water and heat and stir until dissolved.
(1) H medium, per liter

10 g tryptone

8 g NaCl

(2) λ broth medium, per liter
10 g tryptone

2.5 g NaCl

(3) NZC broth medium, per liter

10 g NZ Amine A (NZ Amine A)

5 g NaCl

2 g MgCl₂ - _6H20

High pressure for 30 min

5 ml 20% casein hydrolysate (casamino acid)
(4) LB medium, per liter
10 g tryptone

5 g yeast extract

5 g NaCl

1 ml 1 mol/L NaOH

(5) Super broth medium, per liter

32 g tryptone

20 g yeast extract

5 g NaCl

5 ml 1 mol/L NaOH
(6) TB (super broth medium), per liter
12 g Bacto Tryptone

24 g Bacto yeast extract

4 ml glycerol

Add water to 900 ml and autoclave, add 100 ml of sterile 0. 17 mol/L _KH2PO4 and 0. 72 mol/L _K2HPO4.

(7) Tryptic peptone broth medium per liter.

10 g tryptone

5 g NaCl

(8) 2 X TY medium per liter

15 g tryptone

10 g yeast extract

5 g NaCl

(9) TYGPN medium, per liter

20 g tryptone

10 g yeast extract

10 ml 80% glycerol

5 g _Na2HPO4

10 g _KNO3 2. Pour into a glass vial, loosen the cap and ^autoclave at 15 ^lb/in2 for 25 min.

3. Allow the medium to cool to below 50°C (at this temperature the bottle may feel hot when held in the hand but can be gripped), add the other supplements and antibiotics, cool the glass bottle to below 40°C and close the lid tightly.

4. May be stored indefinitely at room temperature.

For more product details, please visit Aladdin Scientific website.

https://www.aladdinsci.com/