Enzymatic standard curve production experiment
Enzymatic standard curve production experiment
Master the production of enzyme standard curve, the determination of enzyme fixed time method and the use of semi-automated analyzer. This experiment is from Mudanjiang Medical College undergraduate 5-year laboratory guide for testing majors.
Operation method
Enzymatic standard curve production experiment
Principle
L-alanine + α-ketoglutarate ALT pyruvate + L-glutamate pyruvate + NADH + H+ → L-lactate + NAD+ Move Laboratory reagents and instruments: ALT / GPT reagent standards to be measured Semi-automatic biochemical analyzer Test tubes Sampler For more product details, please visit Aladdin Scientific website.
Second, the experimental operation:
1. Dilute the reagents.
2. Turn on the machine and preheat it to 37 degrees Celsius.
3. Select the measurement program.
4. Test:
III. Experimental results: Plot the measured data as ΔA/min-U/L and draw the standard curve.