Dissolve 37.5grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.
Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi)
Component
g/liter
Pancreatic Digest of Gelatin
10
Lactose
10
Dipotassium Phosphate
2
Methylene Blue
0.065
Eosin Y
0.4
Agar
15
Total: 37.5g/Liter
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
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