Experiments on the antagonistic effects of single-salt toxicity and mixed salts

Summary

Monosalt toxicity and antagonism are related to hydrophilic colloids in protoplasm and protoplasmic membranes; the higher the ionic valence, the lower the concentration required to eliminate the toxic effects of the monosalt. Mineral ions, especially cations, have a tremendous influence on the physicochemical properties and physiological functions of protoplasm. When a certain ion alone, often destroy the normal state of the protoplasm and toxic effects; if in the single salt solution, add a small amount of other salts, the antagonistic effect and eliminate the toxic effects.

Operation method

Experiments on the antagonistic effects of single-salt toxicity and mixed salts

Principle

Monosalt toxicity and antagonism are related to hydrophilic colloids in protoplasm and protoplasmic membranes; the higher the ionic valence, the lower the concentration required to eliminate the toxic effects of the monosalt. Mineral ions, especially cations, have a tremendous influence on the physicochemical properties and physiological functions of protoplasm. When a certain ion alone, often destroy the normal state of the protoplasm and toxic effects; if in the single salt solution, add a small amount of other salts, the antagonistic effect and eliminate the toxic effects.

Materials and Instruments

Wheat Rice seedlings
KCl solution NaCl solution CaCl2 solution MgCl2 solution
Beaker Wax cloth Wax paper Measuring cylinder

Move

I. Materials and medicines

1. Materials: wheat or rice seedlings whose true leaves have not yet emerged.

2. Apparatus: 6 beakers; wax cloth or wax paper (the size of which can be masked on the beaker); measuring cylinder;

3. Potions:

(1) 0.12N KCl solution;

(2) 0.12N NaCl solution;

(3) 0.12N CaCl2 solution;

(4) 0.12N MgCl2 solution;

(5) 0.12N NaCl 100 ml + 0.12N CaCl2 1 ml + 0.12N KCl 2.2 ml

(6) 0.12N NaCl 100 ml +0.12N MgCl2 1 ml +0.12N KCl 2.2 ml

(Note: the drug must be of grade A-R)

II. Methods and Steps

1. Take 6 beakers, fill them with the above six solutions and label them .

2. In the center of each sheet of waxed paper, each through an equal distance of 10 small holes, the diameter of the holes and wheat or rice shoots sheath approximate (rather small than large).

3. Select the true leaves are not out of the shoot sheath, equal size, root growth of the same wheat or rice seedlings 60 plants, in each eye of the wax paper planted a plant, (carefully so that the wheat shoot sheath from the bottom up from the small holes) will be covered with waxed paper in 6 beakers, so that the root system of wheat can touch the solution, and then use a thin line to fix the waxed paper, placed in 25-28 ℃ light suitable for the place of cultivation Then fix the wax paper with a thin wire and incubate it at 25-28℃ in a well-lit place, adding distilled water at any time to keep the level of the solution in the cups, observing the results after a week or so, paying special attention to the growth of the root land, recording the lengths of the young roots and stems; and drawing diagrams to show the results.

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