Experiments on the vertebral anesthetic action of procaine
Experiments on the vertebral anesthetic action of procaine
Observation of the performance of the vertebral anesthetic effect of procaine.
Operation method
intravertebral injection
Principle
Intravertebral injection of anesthetics produces different levels of anesthetic effects and is a commonly used method of clinical anesthesia. Intravertebral injection of procaine in rabbits was observed to show the manifestation of vertebral anesthetic effects and to understand the characteristics of procaine vertebral anesthesia.
Materials and Instruments
Rabbit Move 1. Take a healthy rabbit and observe the normal activities. Response of hind limbs to pain and walking gait. Caveat The rabbit body is fixed and the injection site should be accurate. Common Problems Which local anesthetics are used for spinal anesthesia? For which procedures is spinal anesthesia commonly used clinically? For more product details, please visit Aladdin Scientific website.
Procaine hydrochloride injection
Syringe; coarse scissors; alcohol cotton balls, etc.
2. Clip the rabbit's hair in the middle of the lumbosacral region of about 5×5 cm2. The head of the rabbit was clamped under the left armpit of the experimenter, the left hand supported the buttocks, and the rabbit body was fixed on the left forearm and chest, so that the back was arched and the lumbosacral region was flexed as much as possible.
3. Sterilize the skin of the dehairing part of the middle lumbosacral region with alcohol cotton balls. Holding a syringe in the right hand, the syringe was stabbed into the sacral joint space at the lower edge of the spinal process of the seventh lumbar vertebra in the direction of the head, and the animal's hind limb showed a popping response indicating that the needle was stabbed into the vertebral canal, and then injected with 0.3 ml of 2% procaine injection, and the changes of the animal's post-drug status were observed and recorded.
4. record the gait of rabbits after injection of procaine . , the changes in the hind limb response to pain compared to the pre-drug administration and analyzed.