I only have 2.5×10^5 cells in my cell sample, do I only need to use 1.25 μL of flow-through antibody?
Frequently Asked Questions: Products, Experiments and Troubleshooting
I only have 2.5×10^5 cells in my cell sample, do I only need to use 1.25 μL of flow-through antibody?
·Published ·Updated
I only have 2.5×10^5 cells in my cell sample, do I only need to use 1.25 μL of flow-through antibody?
The amount of antibody used is mainly related to the volume of fluid and the number of cells. If the amount of cells is small, the whole system can be halved, that is, 50
μl
cell suspension + 2.5
μl
flow-through antibody. However, if there are very few target cells in the sample, a reduction in the amount of cells may have an effect on the results, resulting in only a small signal being detected.
Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
Cite this article
Aladdin Scientific. "I only have 2.5×10^5 cells in my cell sample, do I only need to use 1.25 μL of flow-through antibody?" Aladdin Knowledge Base, updated 9 jun 2025. https://www.aladdinsci.com/us_es/faqs/flow-through-antibody-en.html
Was this article helpful?YesNo41 out 79 found this helpful
Utilizamos cookies para garantizar el correcto funcionamiento del sitio web y, cuando esté permitido, para mejorar su experiencia. Puede gestionar sus preferencias en cualquier momento en Configuración. Más información en nuestra Política de Cookies.
¿Quieres que te enviemos un mensaje cuando tengamos descuentos disponibles?
Recordármelo más tarde
¡Gracias! Por favor, revisa tu bandeja de entrada de correo electrónico para confirmar.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
