Fluid Thioglycollate Medium w/K Agar (Powder)

Directions per Liter:

Dissolve 29.45g in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Note: After storage of the medium, if 30% of the medium demonstrates oxidation (color change of indicator), reheat the medium once in boiling water or steam bath.

Component	g/liter
L-Cystine	0.5
Dextrose	5
K Agar	0.45
Potassium Chloride	2.5
Resazurin	0.001
Sodium Chloride	0.5
Sodium Thioglycollate	0.5
Casitone	15
Yeast Extract	5
	Total: 29.451g/Liter

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.