IL-10 biological activity assay
IL-10 biological activity assay
In the presence of mouse IL-4 (Mil-4), IL-10 stimulated proliferation of the D36 mouse mast cell line. In contrast, single mIL4 or hIL-10 had only very low proliferation-stimulating activity.
Operation method
IL-10 biological activity assay
Materials and Instruments
Mouse IL-4 Move D36 cell suspension preparation①↓Inoculate cells in a 96-well culture plate②.↓ Caveat ①Take the vigorously growing D36 cells and wash them twice with IMDM culture medium, suspend them in 20% FCS-IMDM culture medium, and make a concentration of 2×104/ml.②~⑥ Refer to IL-4 assay. For more product details, please visit Aladdin Scientific website.
FCS-IMDM culture solution rh IL-10 standard 3H-TdR
Centrifuge Incubator
Add the sample to be tested and the standard ③↓plus 3H-TdR④↓
Cells were collected and the amount of 3H-TdR adulteration was determined ⑤↓Plot the standard curve and calculate the activity units of IL-10 of the samples to be tested ⑥