The surface of living cells retains a more complete antigen or receptor, first use specific mouse-derived monoclonal antibody to bind to the corresponding antigen on the surface of the cell, and then use fluorescently labeled secondary antibody to bind to it, and according to the measured fluorescence intensity and the percentage of positivity, the density and distribution of the corresponding antigen can be known.
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Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
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Cite this article
Aladdin Scientific. "Indirect immunofluorescence of living cells" Aladdin Knowledge Base, updated 24 dic 2024. https://www.aladdinsci.com/us_es/faqs/indirect-immunofluorescence-of-living-ce-en.html
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