Technical articles

Luciferase Enzyme Fact Sheet

· Reviewed · Published 10 ago 2025 · Updated 10 ago 2025 · 8 min read

Luciferase Enzyme Fact Sheet

Source Organism	Species	Mass	Substrate	Wavelength	ATP Dependence
Copepod (Crustacean)	Gaussia princeps	20 kDa	Coelenterazine	460 nm	No
Ostracod (Crustacean)	Cypridina noctiluca	62 kDa	Vargulin (Cypridina Luciferin)	465 nm	No
Sea Pansy	Renilla reniformis	36 kDa	Coelenterazine	480 nm	No
Firefly	Photinus pyralis	62 kDa	D-Luciferin	560 nm	Yes
Click Beetle	Pyrophorus plagiophthalamus	60 kDa	D-Luciferin	Green:537nm Red: 613 nm	Yes

References

Hayat, M. A. (2008). Cancer imaging (Vol. 2). Amsterdam: Elsevier, Academic Press.

Thorne, N., Inglese, J., & Auld, D. S. (2010). Illuminating Insights into Firefly Luciferase and Other Bioluminescent Reporters Used in Chemical Biology. Chemistry & Biology,17(6), 646-657. doi:10.1016/j.chembiol.2010.05.012

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Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Aladdin Scientific. "Luciferase Enzyme Fact Sheet" Aladdin Knowledge Base, updated 10 ago 2025. https://www.aladdinsci.com/us_es/faqs/luciferase-enzyme-fact-sheet-en.html

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