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Modified RIPA Buffer

Protocols

Modified RIPA Buffer

· Reviewed · Published 9 jun 2025 · Updated 27 jul 2023 · 8 min read

Recipe

10 mM Tris, pH 7.4
100 mM NaCl
1 mM EDTA
1 mM EGTA
1 mM NaF
20 mM Na4P2O7
2 mM Na3VO4
1% Triton X-100
10% glycerol
0.1% SDS
0.5% deoxycholate
plus protease inhibitors: Aprotinin, leupeptin, pepstatin: 1ug/ml each
Add 1mM PMSF immediately before use.

Categories: Protocols

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "Modified RIPA Buffer" Aladdin Knowledge Base, updated 27 jul 2023. https://www.aladdinsci.com/us_es/faqs/modified-ripa-buffer-en.html

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