Principles, materials and procedures for PCR amplification and isolation of target DNA fragments

Summary

PCR (Polymerase Chain Reaction) was discovered in 1986 by Kallis Mullis. This technology has been widely used in various fields of molecular biology, not only for gene isolation and cloning and nucleic acid sequence analysis, but also for the construction of mutants and recombinants, the study of the regulation of gene expression, the analysis of gene polymorphisms, genetic and infectious disease diagnosis, tumor mechanism investigation, forensic identification and other aspects. PCR technology has become a revolution in methodology, which will greatly promote the research and development of various disciplines of molecular biology.

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