Dissolve 35.98 grams per liter of distilled/deionized (DDI) water. Heat with stirring until completely solubilized. Adjust pH as necessary. Dispense into appropriate containers. Loosen caps or cover with foil. Autoclave for 15 minutes at 121ºC (15psi).
Component
g/liter
NZ Amine
10
NaCl
10
MgCl2
0.98
Yeast Extract
5
Agar
15
Total: 35.98g/Liter
500g makes 13.9 liters
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
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