Reverse transcription-polymerase chain reaction

Summary

Reverse transcription-polymerase chain reaction (RT-PCR) has improved the sensitivity of RNA detection by several orders of magnitude, making it possible to analyze some extremely minute amounts of RNA samples.

This technique is mainly used for: analyzing transcription products of genes, obtaining target genes, synthesizing cDNA probes, and constructing RNA efficient transcription systems.

Principle

The basic principle of reverse transcription-polymerase chain reaction (RT-PCR) is: extract the total RNA from tissues or cells, use the mRNA as a template, use oligo (dT) or random primers, and use reverse transcriptase to reverse transcribe into cDNA; then use the cDNA as a template for PCR amplification, and then obtain the target genes or detect the expression of genes.

Appliance

Reverse transcription-polymerase chain reaction (RT-PCR) is mainly used for: analyzing transcription products of genes, obtaining target genes, synthesizing cDNA probes, and constructing RNA efficient transcription systems.

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