Fat is soluble in ether, and fat droplets can be recognized by staining.This experiment was derived from the Mudanjiang Medical College undergraduate 5-year clinical laboratory instruction
Operation method
Qualitative urine celiac disease test
Principle
Fats are soluble in ether, while fat droplets can be recognized by staining.
Materials and Instruments
Ether Sudan III Ethyl Acetate Dye Move I. Experimental reagents: Caveat A small amount of 70 g/L NaON followed by ether in the urine helped to clarify the For more product details, please visit Aladdin Scientific website.
Centrifuge
1, ether (AR)
2, Sudan Ⅲ ethanol acetate dye: 5% acetic acid 10 ml. glacial acetic acid 9 ml Sudan Ⅲ powder foam a medicine spoon.
II. Experimental operation
1, take 5-10ml of urine, and 2-3ml of ether, mixing and shaking to make the fat dissolved in ether, and let it stand for a few minutes.
After a few minutes of standing, centrifuge at 2000 γ/min for 5 minutes.
2、Pick up the ether and urine contact layer smear, add 1 drop of Sudan Ⅲ acetic acid ether solution.
3. Observe whether there are red fat droplets by microscopic examination.
Third, the experimental results of judgment:
1. Turbid urine is clarified by the addition of ether to fat or celiac disease.
2. Microscopic examination of red fat droplets.